Murine leukemia provirus-mediated activation of the Notch1 gene leads to induction of HES-1 in a mouse T lymphoma cell line, DL-3
Murine leukemia provirus-mediated activation of the Notch1 gene leads to induction of HES-1 in a mouse T lymphoma cell line, DL-3
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DOI：
10.1016/s0014-5793(99)00901-1
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发表时间：
1999-07-23
期刊：
影响因子：
3.5
通讯作者：
Yanagawa, S
中科院分区：
文献类型：
--
作者：
Lee, JS;Ishimoto, A;Yanagawa, S
在小鼠T淋巴瘤细胞系DL-3中，我们发现一个小鼠白血病前病毒被插入Notch1位点，导致编码Notch1蛋白胞内部分的病毒-Notch1融合mRNA的显著表达，进一步证实了这种组成性活性形式的Notch1蛋白的表达和核定位。与这一发现相对应的是，已知Notch1信号的靶点——分裂促发子（hes1）基因的转录在该细胞系中升高。讨论了过表达HES-1在淋巴瘤发展中的潜在作用。(C) 1999年欧洲生化学会联合会。
Constitutive activation of Notch signaling is known to be associated with tumorigenesis, In a mouse T lymphoma cell line, DL-3, we found that a murine leukemia provirus was inserted in the Notch1 locus, which led to marked expression of a virus-Notch1 fusion mRNA encoding an intracellular portion of the Notch1 protein, Furthermore, expression and nuclear localization of this constitutively active form of Notch1 protein were confirmed. Corresponding to this finding, the transcription of the hairylenhancer of split (HES-1) gene, a known target of Notch1 signaling, mas elevated in this cell line. A potential role for overexpressed HES-1 in the development of the lymphoma was discussed. (C) 1999 Federation of European Biochemical Societies.