Methylated DNA immunoprecipitation (MeDIP) from low amounts of cells.
Methylated DNA immunoprecipitation (MeDIP) from low amounts of cells.
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DOI：
10.1007/978-1-62703-011-3_9
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发表时间：
2012-01-01
期刊：
影响因子：
--
通讯作者：
Weber, Michael
中科院分区：
文献类型：
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作者：
Borgel, Julie;Guibert, Sylvain;Weber, Michael
甲基化DNA免疫沉淀（MeDIP）是一种用于无偏富集胞嘧啶甲基化DNA的免疫捕获方法。其原理是通过超声处理随机剪切基因组DNA，并用特异性识别5-甲基胞苷（5 mC）的抗体进行免疫沉淀，这可以与PCR或高通量分析（微阵列，深度测序）相结合。MeDIP技术最初用于在哺乳动物和植物的基因组规模上生成DNA甲基化谱。在这里，我们提供了一个优化版本的MeDIP协议适用于低量的DNA，它可以用来研究DNA甲基化的细胞群体中可用的少量。
Methylated DNA immunoprecipitation (MeDIP) is an immunocapturing approach for unbiased enrichment of DNA that is methylated on cytosines. The principle is that genomic DNA is randomly sheared by sonication and immunoprecipitated with an antibody that specifically recognizes 5-methylcytidine (5mC), which can be combined with PCR or high-throughput analysis (microarrays, deep sequencing). The MeDIP technique has been originally used to generate DNA methylation profiles on a genome scale in mammals and plants. Here we provide an optimized version of the MeDIP protocol suitable for low amounts of DNA, which can be used to study DNA methylation in cellular populations available in small quantities.