REDUCED LONG-TERM POTENTIATION IN THE DENTATE GYRUS OF TRANSGENIC MICE WITH CEREBRAL OVEREXPRESSION OF INTERLEUKIN-6
REDUCED LONG-TERM POTENTIATION IN THE DENTATE GYRUS OF TRANSGENIC MICE WITH CEREBRAL OVEREXPRESSION OF INTERLEUKIN-6
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DOI：
10.1016/0304-3940(95)11976-4
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发表时间：
1995-09-29
影响因子：
2.5
通讯作者：
SIGGINS, GR
中科院分区：
文献类型：
--
作者：
BELLINGER, FP;MADAMBA, SG;SIGGINS, GR
细胞因子白细胞介素 - 6（IL - 6）可能是几种神经系统疾病中中枢神经系统损伤的一个促成介质。为了研究IL - 6在记忆相关疾病中的作用，我们利用体外制备标本，通过双脉冲易化、双脉冲抑制和长时程增强（LTP）检测了大脑中IL - 6过度表达的转基因小鼠（GFAP - IL6）。我们发现，从GFAP - IL6小鼠制备的海马切片齿状回中的双脉冲增强和抑制与年龄匹配的对照动物没有显著差异，这表明在此模型的体内研究中先前观察到的双脉冲抑制增加是由于来自其他脑区的传入神经改变所致。然而，与同窝野生型对照相比，GFAP - IL6转基因小鼠切片中的齿状回LTP显著降低，这为IL - 6在神经退行性相关记忆障碍的发病机制中的作用提供了支持。
The cytokine interleukin-6 (IL-6) may be a contributing mediator of CNS injury in several neurological disorders. To investigate the role of IL-6 in memory-related disorders, we examined transgenic mice (GFAP-IL6) with cerebral overexpression of IL-6 using paired-pulse facilitation, paired-pulse inhibition, and-long-term potentiation (LTP) in an in vitro preparation. We found that paired-pulse potentiation and inhibition in the dentate gyrus of hippocampal slices prepared from the GFAP-IL6 mice did not differ significantly from age-matched control animals, suggesting that the increase in paired-pulse inhibition seen previously in in vivo studies of this model was due to alterations of afferents from other brain regions. However, LTP in the dentate was significantly reduced in slices from GFAP-IL6 transgenic mice when compared with littermate wild-type controls, providing support for a role of IL-6 in the pathogenesis of neurodegenerative associated memory-related disorders.