Multichannel MRI Labeling of Mammalian Cells by Switchable Nanocarriers for Hyperpolarized Xenon
Multichannel MRI Labeling of Mammalian Cells by Switchable Nanocarriers for Hyperpolarized Xenon
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DOI：
10.1021/nl502498w
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发表时间：
2014-10-01
期刊：
影响因子：
10.8
通讯作者：
Schroeder, Leif
中科院分区：
文献类型：
--
作者：
Klippel, Stefan;Freund, Christian;Schroeder, Leif
我们展示了使用封装的激光偏振氙气进行多通道MRI细胞标记的概念。两种纳米载体在概念上不同的Xe捕获特性，即作为单独宿主的大环笼或区隔成纳米液滴，确保了即使在细胞内化后，在任何一种载体中结合的Xe都有很大的化学位移分离。两种不同标记的哺乳动物细胞群通过频率选择饱和转移成像，在微至纳摩尔的Xe载体浓度下产生可切换的双色氙- mri对比。
We demonstrate a concept for multichannel MRI cell-labeling using encapsulated laser-polarized xenon. Conceptually different Xe trapping properties of two nanocarriers, namely macrocyclic cages as individual hosts or compartmentalization into nanodroplets, ensure a large chemical shift separation for Xe bound in either of the carriers even after cellular internalization. Two differently labeled mammalian cell populations were imaged by frequency selective saturation transfer resulting in a switchable two-color xenon-MRI contrast at micro- to nanomolar Xe carrier concentrations.