Using BOLD Imaging to Measure Renal Oxygenation Dynamics in Rats Injected with Diuretics
Using BOLD Imaging to Measure Renal Oxygenation Dynamics in Rats Injected with Diuretics
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DOI：
10.2463/mrms.9.187
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发表时间：
2010-01-01
影响因子：
3
通讯作者：
Murase, Kenya
中科院分区：
文献类型：
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作者：
Kusakabe, Yoshinori;Matsushita, Taro;Murase, Kenya
目的：我们用血氧水平依赖的磁共振成像（BOLD MRI），以衡量肾氧合动力学在大鼠注射利尿剂和评估利尿剂对肾oxygenation.Methods的影响：我们进行了BOLD MRI研究在32只大鼠使用1.5特斯拉磁共振成像系统的动物实验。我们给大鼠静脉注射生理盐水（n = 7）、呋塞米（n = 7）、乙酰唑胺（n = 6）或甘露醇（n = 6）。对于对照组，6只大鼠未注射药物。我们从表观横向弛豫时间（T（2）*）加权图像估计表观横向弛豫率（R（2）*），并在大约30分钟内以4分钟的间隔测量R（2）* 的时间过程。与给药前值相比，R（2）* 对照组和甘露醇组皮质和髓质中的值均无显著变化，但生理盐水组显著降低;速尿组R（2）* 值在髓质显著降低，皮质无显著变化;乙酰唑胺组R（2）* 值在髓质显著升高，皮质显著降低。我们的研究结果表明，BOLD MRI可用于评估肾皮质和肾脏中对各种利尿剂反应的肾氧合动力学，骨髓
Purpose: We used blood oxygenation level-dependent magnetic resonance imaging (BOLD MRI) to measure renal oxygenation dynamics in rats injected with diuretics and evaluated diuretic effect on renal oxygenation.Methods: We performed BOLD MRI studies in 32 rats using a 1.5-tesla MR imaging system for animal experiments. We intravenously injected rats with saline (n = 7), furosemide (n = 7), acetazolamide (n = 6), or mannitol (n = 6). For controls, 6 rats were not injected with drugs. We estimated the apparent transverse relaxation rate (R(2)*) from the apparent transverse relaxation time (T(2)*)-weighted images and measured the time course of R(2)* at 4-min intervals over approximately 30 min.Results: Compared with preadministration values, the R(2)* value did not change significantly in either the cortex or medulla in the control and mannitol groups but decreased significantly in the saline group; the R(2)* value significantly decreased in the medulla but did not change significantly in the cortex in the furosemide group; and the R(2)* value significantly increased in the medulla and significantly decreased in the cortex in the acetazolamide group.Conclusion: Our study results suggest that BOLD MRI is useful for evaluating the dynamics of renal oxygenation in response to various diuretics in the renal cortex and in the medulla.