Maternal Obesity Enhances White Adipose Tissue Differentiation and Alters Genome-Scale DNA Methylation in Male Rat Offspring
Maternal Obesity Enhances White Adipose Tissue Differentiation and Alters Genome-Scale DNA Methylation in Male Rat Offspring
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DOI：
10.1210/en.2012-2255
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发表时间：
2013-11-01
期刊：
影响因子：
4.8
通讯作者：
Shankar, Kartik
中科院分区：
文献类型：
--
作者：
Borengasser, Sarah J.;Zhong, Ying;Shankar, Kartik
成年期肥胖（OB）的风险受到母体身体成分的强烈影响。在这里，我们使用一个已建立的长期代谢程序模型，检验了母体OB在OB发育之前影响白色脂肪组织（WAT）转录组并增加后代脂肪形成倾向的假设。采用过度喂养大鼠模型，其中暴露于OB仅限于孕前和妊娠期，我们对WAT进行了全球转录组学分析，并对脂肪生成和脂肪生成途径进行了基因/蛋白质表达分析，并检查了WAT基质血管细胞的体外脂肪分化。使用亚硫酸盐还原测序，我们还评估了后代WAT中DNA甲基化的基因组尺度变化。母体OB导致基因表达的广泛变化(+/-1.8倍，P
The risk of obesity (OB) in adulthood is strongly influenced by maternal body composition. Here we examined the hypothesis that maternal OB influences white adipose tissue (WAT) transcriptome and increases propensity for adipogenesis in the offspring, prior to the development of OB, using an established model of long-term metabolic programming. Employing an overfeeding-based rat model, in which exposure to OB is limited to preconception and gestation alone, we conducted global transcriptomic profiling in WAT, and gene/protein expression analysis of lipogenic and adipogenic pathways and examined adipogenic differentiation of WAT stromal-vascular cells ex vivo. Using reduced representation bisulfite sequencing we also evaluated genome-scale changes in DNA methylation in offspring WAT. Maternal OB led to extensive changes in expression of genes (+/-1.8-fold, P