MYOGENIC CELL LINE WITH ALTERED SERUM REQUIREMENTS FOR DIFFERENTIATION
MYOGENIC CELL LINE WITH ALTERED SERUM REQUIREMENTS FOR DIFFERENTIATION
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DOI：
10.1111/j.1432-0436.1977.tb01507.x
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发表时间：
1977-01-01
期刊：
影响因子：
2.9
通讯作者：
SAXEL, O
中科院分区：
文献类型：
--
作者：
YAFFE, D;SAXEL, O
描述了[大鼠]细胞系L84的分化特性，该特性源自从肌源性线L8分离出的非合格克隆。在补充有L8细胞的10％血清的营养培养基中，L84细胞继续增殖至非常高的密度，未能形成多核纤维。当在补充2％马血清或2％马血清加0.1 .mu.g/ml胰岛素的培养基中生长时，L84细胞的表现与在补充10％马血清的培养基中生长的L8细胞非常相似：当培养物达到融合时，减少，细胞开始融合并形成密集的纤维网络。肌酸激酶活性和肌球蛋白的合成大大增加与细胞融合有关。在L84细胞未融合的条件下，即使达到了极高的细胞密度，也没有观察到这些合成活性的增加。 L84细胞保留了该程序以分化为肌肉纤维。 L84及其祖细胞线L8之间的差异在对触发该程序表达的环境条件的敏感性中。
Differentiation properties of a [rat] cell line, L84, which originated from a non-fusing clone isolated from the myogenic line L8, are described. In nutritional medium supplemented with 10% serum used routinely with L8 cells, L84 cells continued to proliferate to very high densities and failed to form multinucleated fibers. When grown in medium supplemented with 2% horse serum or 2% horse serum plus 0.1 .mu.g/ml insulin, L84 cells behaved very similarly to L8 cells grown in medium supplemented with 10% horse serum: when the cultures reached confluency, proliferation decreased and cells started to fuse and formed a dense network of fibers. Large increases in creatine kinase activity and synthesis of myosin were associated with cell fusion. Under conditions in which L84 cells did not fuse the increase in these synthetic activities was not observed, even after extremely high cell densities were reached. L84 cells retained the program for their differentiation into muscle fibers. The difference between L84 and its progenitor line L8 laid in the sensitivity to the environmental conditions which trigger the expression of this program.