ALPHA-TUBULIN-II IS A MALE-SPECIFIC PROTEIN IN PLASMODIUM-FALCIPARUM
ALPHA-TUBULIN-II IS A MALE-SPECIFIC PROTEIN IN PLASMODIUM-FALCIPARUM
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DOI：
10.1016/0166-6851(92)90173-h
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发表时间：
1992-12-01
影响因子：
1.5
通讯作者：
KASLOW, DC
中科院分区：
文献类型：
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作者：
RAWLINGS, DJ;FUJIOKA, H;KASLOW, DC
恶性疟原虫中的微管蛋白基因家族由一个β-微管蛋白和两个α-微管蛋白基因（α-微管蛋白I和II）组成。我们在这里提出的数据表明，α-微管蛋白II只在男性有性阶段的寄生虫。IgM mAb 5E 7通过成熟（第10-11天）雄性配子母细胞与III期（第4-5天）特异性反应，并与新兴、脱鞭毛或自由移动的雄性配子特异性反应。在雌性配子母细胞、雌性配子、子孢子或无性寄生虫中未检测到反应性。mAb 5E 7还特异性识别禽类寄生虫鸡疟原虫的雄配子，并在来自两个物种的雄配子提取物中免疫印迹50 kDa蛋白。这50 kDa的抗原通过免疫电子显微镜定位到轴丝的男性配子的模式类似的抗α-和抗β-微管蛋白抗体获得。此外，mAb 5E 7与使用来自配子体特异性cDNA文库的PCR扩增的α-微管蛋白II基因获得的重组α-微管蛋白II蛋白特异性反应。性别特异性表达的α-微管蛋白II和其本地化的轴丝的男性寄生虫的形态变化，发生在出鞭毛和寄生虫的运动性的分子中建议的作用。α-微管蛋白II和单克隆抗体5E 7可能被证明是有用的工具，在恶性疟原虫的性阶段分化和发育的生物学研究，除了一般的理解微管蛋白异构体的翻译后修饰。
The tubulin gene family in Plasmodium falciparum consists of one beta-tubulin and two alpha-tubulin genes (alpha-tubulin I and II). We present here data indicating that alpha-tubulin II is expressed only in male sexual stage parasites. An IgM mAb, 5E7, specifically reacted with stage III (day 4-5) through mature (day 10-11) male gametocytes and with emerging, exflagellating, or freely moving male gametes. No reactivity was detected in female gametocytes, female gametes, sporozoites, or asexual parasites. mAb 5E7 also specifically recognized male gametes of the avian parasite, Plasmodium gallinaceum, and immunoblotted a 50 kDa protein in extracts of male gametes from both species. This 50 kDa antigen was localized by immunoelectron microscopy to axonemes of male gametes in a pattern similar to that obtained with anti-alpha- and anti-beta-tubulin antibodies. Furthermore, mAb 5E7 specifically reacted with recombinant alpha-tubulin II protein obtained using the PCR-amplified alpha-tubulin II gene from a gametocyte-specific cDNA library. The sex-specific expression of alpha-tubulin II and its localization to axoneme of the male parasite suggest a role for this molecule in the morphologic changes that occur during exflagellation and in the motility of the parasite. alpha-Tubulin II and mAb 5E7 may prove useful tools in studies of the biology of sexual stage differentiation and development in P. falciparum in addition to the general understanding of post-translational modifications of tubulin isoforms.