daf-7 and the development of Strongyloides ratti and Parastrongyloides trichosuri
daf-7 and the development of Strongyloides ratti and Parastrongyloides trichosuri
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DOI：
10.1016/j.molbiopara.2004.11.010
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发表时间：
2005-02-01
影响因子：
1.5
通讯作者：
Viney, ME
中科院分区：
文献类型：
--
作者：
Crook, M;Thompson, FJ;Viney, ME
daf-7是控制秀丽隐杆线虫幼虫发育的三条途径之一的关键配体。鉴于自由生活线虫的dauer幼虫和动物寄生线虫的第三阶段感染性幼虫之间的相似性，我们假设daf-7可能参与这些感染性幼虫的发育。为了研究这一点，我们克隆了来自拉氏圆线虫和苏氏副圆线虫的daf-7直向同源物，并通过半定量RT-PCR分析了它们在S. Ratti和P. lastsuri生命周期以及在一系列体外和体内条件下。我们发现，在这两个物种中，daf-7的RNA水平在自由生活阶段较低，但在感染L3（iL 3）阶段达到峰值，在寄生阶段很少或没有表达。这与C. elegans，在L1中达到峰值，此后减少，并且在Dauer幼虫中不存在。感染性幼虫中daf-7的RNA水平通过幼虫穿透宿主皮肤或在宿主中发育而降低，但不通过转移到宿主的体温而降低。(C)2004 Elsevier B. V.保留所有权利。
daf-7 is a key ligand in one of the three pathways that control dauer larva development in Caenorhabditis elegans. Given the similarities between dauer larvae of free-living nematodes and third stage infective larvae of animal parasitic nematodes, we hypothesised that daf-7 may be involved in the development of these infective larvae. To investigate this, we cloned daf-7 orthologues from Strongyloides ratti and Parastrongyloides trichosuri and analysed their RNA level by semi-quantitative RT-PCR during the S. ratti and P. trichosuri life cycles and in a range of in vitro and in vivo conditions. We found that, in both species, the RNA level of daf-7 was low in free-living stages but peaked in the infective L3 (iL3) stage with little or no expression in the parasitic stages. This contrasts with the daf-7 RNA level in C. elegans, which peaks in L 1, decreases thereafter, and is absent in dauer larvae. The RNA level of daf-7 in infective larvae was reduced by larval penetration of host skin or development in the host, but not by a shift to the body temperature of the host. (C) 2004 Elsevier B.V. All rights reserved.