Organoid generation from mouse mammary tumors captures the genetic heterogeneity of clinically relevant copy number alterations.
Organoid generation from mouse mammary tumors captures the genetic heterogeneity of clinically relevant copy number alterations.
复制标题
小鼠乳腺肿瘤的类器官生成捕获了临床相关拷贝数改变的遗传异质性。
DOI：
10.1101/2023.01.29.526141
复制
发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Chan,IsaacS
中科院分区：
文献类型：
--
作者：
Lake,KatherineE;Colonetta,MeganM;Smith,ClaytonA;Martinez-Algarin,Kenneth;Saunders,Kaitlyn;Mohta,Sakshi;Pena,Jacob;McArthur,HeatherL;Reddy,SangeethaM;Roussos-Torres,EvanthiaT;Chen,ElizabethH;Chan,IsaacS
乳腺癌转移表现出许多不同的遗传改变，包括拷贝数扩增。使用公开可用的数据集，我们确定了转移性乳腺肿瘤样本中的拷贝数扩增，并使用我们的基于类器官的转移测定，我们验证了FGFR1在集体迁移的类器官中扩增。由于乳腺肿瘤的异质性越来越与临床实践相关，我们证明了我们的类器官方法捕获了个体肿瘤的遗传异质性。
Breast cancer metastases exhibit many different genetic alterations, including copy number amplifications. Using publicly available datasets, we identify copy number amplifications in metastatic breast tumor samples and using our organoid-based metastasis assays, and we validate FGFR1 is amplified in collectively migrating organoids. Because the heterogeneity of breast tumors is increasingly becoming relevant to clinical practice, we demonstrate our organoid method captures genetic heterogeneity of individual tumors.