LGR4 Is Required for the Cell Survival of the Peripheral Mesenchyme at the Embryonic Stages of Nephrogenesis
LGR4 Is Required for the Cell Survival of the Peripheral Mesenchyme at the Embryonic Stages of Nephrogenesis
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DOI：
10.1271/bbb.110834
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发表时间：
2012-05
期刊：
影响因子：
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通讯作者：
Y. Mohri;Kazunori Oyama;Mizuki Sone;Atsushi Akamatsu;K. Nishimori
中科院分区：
文献类型：
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作者：
Y. Mohri;Kazunori Oyama;Mizuki Sone;Atsushi Akamatsu;K. Nishimori
在小鼠中，纯合Lgr 4失活导致肾发育不良。为了更好地理解LGR 4在肾脏发育中的作用，我们对LGR 4-/-胚胎中的肾脏进行了分析。我们在E16.5用抗WT 1和抗Cleaved Caspase 3抗体对Lgr 4-/-肾进行染色，观察到帽间充质结构被破坏，细胞凋亡增加。此外，肾脏发育中的抗凋亡因子PAX 2的表达在E16.5时也显著降低。我们发现LGR 4缺陷导致肾脏发育过程中外周间充质细胞凋亡增加。
In mice, homozygous Lgr4 inactivation results in hypoplastic kidneys. To understand better the role of LGR4 in kidney development, we performed an analysis of kidneys in Lgr4 -/- embryos. We stained Lgr4 -/- kidneys with anti-WT1 and anti-Cleaved Caspase3 antibodies at E16.5, and observed that the structures of the cap mesenchyme were disrupted and that apoptosis increased. In addition, the expression of PAX2, an anti-apoptotic factor in kidney development, was also significantly decreased at E16.5. We found that the LGR4 defect caused an increase in apoptosis in the peripheral mesenchyme during kidney development.