PROMOTER RECOGNITION AND PROMOTER STRENGTH IN THE ESCHERICHIA-COLI SYSTEM
PROMOTER RECOGNITION AND PROMOTER STRENGTH IN THE ESCHERICHIA-COLI SYSTEM
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DOI：
10.1002/j.1460-2075.1987.tb02624.x
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发表时间：
1987-10-01
期刊：
影响因子：
11.4
通讯作者：
BUJARD, H
中科院分区：
文献类型：
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作者：
BRUNNER, M;BUJARD, H
大肠杆菌启动子在体内的强度以及RNA聚合酶和启动子序列之间的结合速率相差超过一个数量级。由于有效的启动子识别和酶的快速结合可能是特殊启动子强度的先决条件，我们已经确定了9个启动子的正向速率常数Kon（以及koff），这些启动子包括分别来自λ，T7和T5的PL，PA 1和PN 25，以及来自E.杆菌二阶正向速率常数跨越从1 × 10 - 4到10 - 4的30倍范围。Pbla和PL高达2.9 × 107 M-1 s-1。108 M-1，用于PN 25。已经发现，由启动子序列与RNA聚合酶的复合物形成速率定义的“启动子增强”与由RNA合成速率定义的其体内强度之间几乎没有相关性。这增加了以下证据：启动子序列中编码的复杂功能途径可以在各种水平上受到限制，并且体内启动子强度是涉及更多的优化过程的结果。而不仅仅是一个功能参数。
The strength of Escherichia coli promoters in vivo as well as the rate of association between RNA polymerase and promoter sequences differ by more than an order of magnitude. Since efficient promoter recognition and rapid binding of the enzyme might be a prerequisite for exceptional promoter strength we have determined the forward rate constants Kon (as well as koff) for nine promoters including PL, PA1, and PN25 from phages lambda, T7, and T5, respectively as well as Pbla and PlacUV5 from E. coli. The second order forward rate constants span a 30-fold range from 1 .times. 107 M-1 s-1 for Pbla and PL up to 2.9 .times. 108 M-1 for PN25. Little correlation between ''promoter recognition'' as defined by the rate of complex formation of a promoter sequence with RNA polymerase and its strength in vivo as defined by the rate of RNA synthesis has been found. This adds to the evidence that the complex functional pathway encoded in a promoter sequence can be limited at various levels and that promoter strength in vivo is the result of an optimization process involving more than just one functional parameter.