Brain immune cell composition and functional outcome after cerebral ischemia: comparison of two mouse strains.
Brain immune cell composition and functional outcome after cerebral ischemia: comparison of two mouse strains.
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DOI：
10.3389/fncel.2014.00365
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发表时间：
2014
影响因子：
5.3
通讯作者：
Sobey CG
中科院分区：
文献类型：
--
作者：
Kim HA;Whittle SC;Lee S;Chu HX;Zhang SR;Wei Z;Arumugam TV;Vinh A;Drummond GR;Sobey CG
炎性细胞可能参与了脑缺血后继发性脑损伤。已知C57BL/6小鼠株对损伤表现出T辅助细胞1倾向于促炎性类型的反应，而FVB株在其免疫反应中相对容易T辅助细胞2或抗炎。我们测试了C57BL/6小鼠的中风结局是否比FVB小鼠更严重。每种品系的雄性小鼠均行假手术或大脑中动脉阻断1h，然后再灌注23h。尽管梗塞面积没有差异，但根据神经学评分和挂线试验，C57BL/6小鼠在中风后表现出明显比FVB小鼠更大的功能缺陷。C57BL/6小鼠中风后脑内CD45+白细胞总数较FVB小鼠明显增多，但两种品系小鼠的白细胞组成有显著差异。C57BL/6小鼠炎症反应主要以T、B淋巴细胞为主，而FVB小鼠以中性粒细胞、单核细胞和巨噬细胞为主。我们的数据与中风后的功能结果取决于缺血性脑损伤后形成的免疫细胞成分的概念是一致的。
Inflammatory cells may contribute to secondary brain injury following cerebral ischemia. The C57Bl/6 mouse strain is known to exhibit a T helper 1-prone, pro-inflammatory type response to injury, whereas the FVB strain is relatively T helper 2-prone, or anti-inflammatory, in its immune response. We tested whether stroke outcome is more severe in C57Bl/6 than FVB mice. Male mice of each strain underwent sham surgery or 1 h occlusion of the middle cerebral artery followed by 23 h of reperfusion. Despite no difference in infarct size, C57Bl/6 mice displayed markedly greater functional deficits than FVB mice after stroke, as assessed by neurological scoring and hanging wire test. Total numbers of CD45+ leukocytes tended to be larger in the brains of C57Bl/6 than FVB mice after stroke, but there were marked differences in leukocyte composition between the two mouse strains. The inflammatory response in C57Bl/6 mice primarily involved T and B lymphocytes, whereas neutrophils, monocytes and macrophages were more prominent in FVB mice. Our data are consistent with the concept that functional outcome after stroke is dependent on the immune cell composition which develops following ischemic brain injury.
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影响因子：
82.9
作者：
Liesz, Arthur;Suri-Payer, Elisabeth;Veltkamp, Roland
通讯作者：
Veltkamp, Roland
影响因子：
8.3
作者：
Gelderblom, Mathias;Leypoldt, Frank;Magnus, Tim
通讯作者：
Magnus, Tim
影响因子：
2.9
作者：
Brait, Vanessa H.;Rivera, Jennifer;Sobey, Christopher G.
通讯作者：
Sobey, Christopher G.
DOI：
10.1523/jneurosci.3257-09.2009
发表时间：
2009-10-28
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Kigerl KA;Gensel JC;Ankeny DP;Alexander JK;Donnelly DJ;Popovich PG
通讯作者：
Popovich PG
影响因子：
7.3
作者：
Jackman, K. A.;Miller, A. A.;Sobey, C. G.
通讯作者：
Sobey, C. G.