Cloning and expression of Hoxc6 gene from Pampus argenteus and its relationship with pelvic fin absence
Cloning and expression of Hoxc6 gene from Pampus argenteus and its relationship with pelvic fin absence
复制标题
银鲳Hoxc6基因的克隆、表达及其与腹鳍缺失的关系
DOI：
10.1016/j.gep.2020.119161
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发表时间：
2020
影响因子：
1.2
通讯作者：
Danli Wang
中科院分区：
文献类型：
--
作者：
Lingzhu Hu;Shun Zhang;Yu Zhou;Kai Liao;Shanliang Xu;Danli Wang
Hoxc6基因可以被描述为在早期胚胎发生中的轴向模式中起作用，并且至少在某些物种中，对肢体定位有贡献。在这项研究中，我们克隆和表征Pampus argenteus Hoxc6。高度保守的HOXC 6蛋白序列包含一个同源结构域和一个低复杂性区域。实时荧光定量PCR检测到Hoxc6 mRNA在不同发育阶段和不同组织中的表达（p < 0.05），在眼囊和脑分化阶段表达量较高，而在7日龄和13日龄幼虫中表达量较低。Hoxc6 mRNA在鳍组织中的表达量高于脑和眼组织。Western blotting结果显示，HOXC 6蛋白在胚胎期表达量较高，而在7、13、16和19日龄幼虫中表达量显著下降，且与实时荧光定量PCR检测的mRNA在不同组织中的表达量基本一致。原位杂交结果表明，Hoxc6转录本在1日龄仔鱼的全脑和体轴前部强烈表达，而在7、13、16和19日龄仔鱼的后脑、胸鳍、下颌骨和假设的腹鳍区域表达。这些结果阐明了Hoxc6基因在银鲳中的表达和定位特征，为银腹鳍缺失的分子机制提供了理论依据。
Hoxc6 gene can be described as having roles in axial patterning in early embryogenesis, and in at least some species, having a contribution to limb positioning. In this study, we cloned and characterised Pampus argenteus Hoxc6. The highly conserved HOXC6 protein sequence contains a homeodomain and a low-complexity region. Expression of Hoxc6 mRNA was measured at different developmental stages and in different tissues by real-time PCR (p < 0.05), and was high during eye capsule and brain differentiation stages, but low in 7 and 13-day-old larvae. Hoxc6 mRNA was more abundant in fin tissue than brain and eye tissues. Western blotting showed that HOXC6 protein levels were high at embryonic stages, but decreased significantly in 7, 13, 16 and 19-day-old larvae, and levels were essentially consistent with those of mRNA measured by real-time PCR in different tissues. In situ hybridisation showed that the Hoxc6 transcript was strongly expressed in the whole brain and anterior part of the body axis in 1-day-old larvae, but in the hindbrain, pectoral fin, mandible and hypothetical pelvic fin region in 7, 13, 16 and 19-day-old organisms. These results clarify the expression and localisation characteristics of Hoxc6 gene in P. argenteus, and provide a theoretical basis for the molecular mechanism of pelvic fin loss in silver pomfret.