Calibrant-Free Analyte Quantitation via a Variable Velocity Flow Cell.
Calibrant-Free Analyte Quantitation via a Variable Velocity Flow Cell.
复制标题
DOI：
10.1021/acs.analchem.6b03527
复制
发表时间：
2017-01-17
影响因子：
7.4
通讯作者：
Porter MD
中科院分区：
文献类型：
--
作者：
Beck JG;Skuratovsky A;Granger MC;Porter MD
在本文中，我们描述了一种新的分析物定量方法，该方法不依赖于校准剂，内部标准或校准曲线，而是利用不同的和可预测的表面定向分析物通量到传感地址阵列和测量结果信号之间的关系。为了将这一概念付诸实践，我们制造了两个流动池，使平均线性流体速度U在沿流动轴放置的电极阵列上系统地变化。这导致氧化还原分析物二茂铁二甲醇（FDM）的定向通量的可预测变化。在一系列这些电极上测量所得的极限电流，并通过对流扩散输运模型精确描述，提供了一种计算“未知”浓度的方法，而无需使用校准剂、内部标准或校准曲线。此外，实验和浓度计算只需几分钟即可完成。当使用经验推导的U值时，计算出的FDM浓度与真实值的偏差被最小化到小于0.5%。
In this paper, we describe a novel method for analyte quantitation that does not rely on calibrants, internal standards, or calibration curves but, rather, leverages the relationship between disparate and predictable surface-directed analyte flux to an array of sensing addresses and a measured resultant signal. To reduce this concept to practice, we fabricated two flow cells such that the mean linear fluid velocity, U, was varied systematically over an array of electrodes positioned along the flow axis. This resulted in a predictable variation of the address-directed flux of a redox analyte, ferrocenedimethanol (FDM). The resultant limiting currents measured at a series of these electrodes, and accurately described by a convective-diffusive transport model, provided a means to calculate an “unknown” concentration without the use of calibrants, internal standards, or a calibration curve. Furthermore, the experiment and concentration calculation only takes minutes to perform. Deviation in calculated FDM concentrations from true values was minimized to less than 0.5% when empirically derived values of U were employed.