A regenerative approach towards recovering the mechanical properties of degenerated intervertebral discs: Genipin and platelet-rich plasma therapies
A regenerative approach towards recovering the mechanical properties of degenerated intervertebral discs: Genipin and platelet-rich plasma therapies
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DOI：
10.1177/0954411916681597
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发表时间：
2017-02-01
期刊：
影响因子：
1.8
通讯作者：
Khalaf, Kinda
中科院分区：
文献类型：
--
作者：
Nikkhoo, Mohammad;Wang, Jaw-Lin;Khalaf, Kinda
椎间盘退行性疾病与外周环和椎体终板的离散结构改变有关，是急性和慢性腰痛最常见的病理诱因之一，显著降低了个体的生活质量，并引发了巨大的社会经济成本。因此，新兴的治疗技术引起了研究和临床界的极大兴趣。外源性交联疗法，如Genipin和富血小板血浆疗法，最近在退变椎间盘的修复和再生方面显示出令人鼓舞的结果，但关于有效性的定量程度和对椎间盘力学性能的特殊影响，仍然存在知识空白。本研究旨在研究和量化32只猪胸部运动节段的完整（N=8）、胰蛋白酶变性（N=8）、genipin处理（N=8）和富血小板血浆处理（N=8）椎间盘的材料特性。采用孔弹性有限元模型描述不同处理下的力学性能，采用元模型分析方法结合离体实验提取孔弹性材料的力学性能。结果显示，Genipin和富血小板血浆都能恢复变性椎间盘的机械性能，从而提供了有希望的治疗方式。然而，在恢复糖胺聚糖含量方面，富血小板血浆处理的椎间盘表现略好，但并不明显，优于Genipin，糖胺聚糖是健康椎间盘的重要组成部分。除了研究这些特殊的退行性椎间盘疾病治疗方法外，本研究还提供了一种系统的方法来量化椎间盘的详细孔隙弹性力学特性。
Degenerative disc disease, associated with discrete structural changes in the peripheral annulus and vertebral endplate, is one of the most common pathological triggers of acute and chronic low back pain, significantly depreciating an individual's quality of life and instigating huge socioeconomic costs. Novel emerging therapeutic techniques are hence of great interest to both research and clinical communities alike. Exogenous crosslinking, such as Genipin, and platelet-rich plasma therapies have been recently demonstrated encouraging results for the repair and regeneration of degenerated discs, but there remains a knowledge gap regarding the quantitative degree of effectiveness and particular influence on the mechanical properties of the disc. This study aimed to investigate and quantify the material properties of intact (N=8), trypsin-denatured (N=8), Genipin-treated (N=8), and platelet-rich plasma-treated (N=8) discs in 32 porcine thoracic motion segments. A poroelastic finite element model was used to describe the mechanical properties during different treatments, while a meta-model analytical approach was used in combination with ex vivo experiments to extract the poroelastic material properties. The results revealed that both Genipin and platelet-rich plasma are able to recover the mechanical properties of denatured discs, thereby affording promising therapeutic modalities. However, platelet-rich plasma-treated discs fared slightly, but not significantly, better than Genipin in terms of recovering the glycosaminoglycans content, an essential building block for healthy discs. In addition to investigating these particular degenerative disc disease therapies, this study provides a systematic methodology for quantifying the detailed poroelastic mechanical properties of intervertebral disc.