The correlation between LIN28B gene potentially functional variants and Wilms tumor susceptibility in Chinese children
The correlation between LIN28B gene potentially functional variants and Wilms tumor susceptibility in Chinese children
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DOI：
10.1002/jcla.22200
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发表时间：
2018-01-01
影响因子：
2.7
通讯作者：
Xia, Huimin
中科院分区：
文献类型：
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作者：
Fu, Wen;Liu, Guo-Chang;Xia, Huimin
肾母细胞瘤(Wilms Tumor，WT)是儿童常见的泌尿系肿瘤。然而，WT背后的遗传基础在很大程度上仍不清楚。以往的研究表明，在一些WTS中，Lin28同源基因B(Lin28b)水平显著升高。Lin28b在肾脏发育过程中的表达增强可诱发小鼠肾移植。Lin28b基因的遗传变异可能与WT易感性有关。方法本研究旨在探讨Lin28b基因多态性与WT易感性的关系。采用Taqman法对145例肺癌患者和531例正常对照的Lin28b基因4个潜在功能多态(rs314276C>；A、rs221634A>；T、rs221635T>；C和rs9404590T>；G)进行了基因分型。结果rs314276 CA基因型与WT风险降低相关(CA与CC：调整后OR=0.65，95%CI=0.43~0.98，P=0.042)。此外，我们还发现，与非携带者相比，携带1-3个风险基因的携带者WT风险显著增加(调整后的OR=1.51，95%CI=1.03-2.20，P=0.035)。结论Lin28b基因rs314276C>；A单基因多态和3个联合多态可能影响华南地区儿童WT的易感性。我们的发现需要在涉及不同种族的大型研究中进一步验证。
BackgroundWilms tumor (WT) is the most common urologic cancer in children. However, genetic bases underlying WT remain largely unknown. Previous studies indicated that Lin28 homolog B (LIN28B) level is significantly elevated in some WTs. Enforced expression of Lin28b during kidney development could induce WT. Genetic variations in the LIN28B gene may be related to WT susceptibility.MethodIn this study, we aimed to assess the association between LIN28B gene polymorphisms and WT susceptibility in Chinese children. Four potentially functional polymorphisms in the LIN28B gene (rs314276 C>A, rs221634 A>T, rs221635 T>C and rs9404590 T>G) were genotyped in 145 cases and 531 cancer-free controls, using Taqman method. Odds ratios (ORs) and 95% confidence intervals (CIs) were calculated to evaluate the strength of the associations.ResultsOur results showed that the rs314276 CA genotype was associated with a decreased WT risk (CA vs CC: adjusted OR=0.65, 95% CI=0.43-0.98, P=.042). Moreover, we found that carriers of the 1-3 risk genotypes had a significantly increased WT risk when compared to the non-carriers (adjusted OR=1.51, 95% CI=1.03-2.20, P=.035). The association with risk genotypes was more predominant in children 18month old or younger and in females.ConclusionIn summary, these results indicated that the LIN28B gene rs314276 C>A polymorphism alone and three combined polymorphisms may be able to modify WT susceptibility in Southern Chinese children. Our findings call for further validation in large studies with different ethnicities involved.