Insecticidal activity of two proteases against Spodoptera frugiperda larvae infected with recombinant baculoviruses.
Insecticidal activity of two proteases against Spodoptera frugiperda larvae infected with recombinant baculoviruses.
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DOI：
10.1186/1743-422x-7-143
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发表时间：
2010-06-29
期刊：
影响因子：
4.8
通讯作者：
Ribeiro BM
中科院分区：
文献类型：
--
作者：
Gramkow AW;Perecmanis S;Sousa RL;Noronha EF;Felix CR;Nagata T;Ribeiro BM
杆状病毒是世界上研究最多的昆虫病毒，主要是因为它们能有效地杀死农业害虫。在这项研究中，两个重组杆状病毒含有ScathL基因从Sarcophaga peregrina（vSynScathL），角蛋白酶基因从真菌烟曲霉（vSynKerat），构建。对草地贪夜蛾幼虫的杀虫活性进行了测定。三龄幼虫和新生幼虫的生物测定。当与野生型病毒相比时，用vSynScathL和vSynKerat处理的草地贪夜蛾幼虫显示出杀死感染的昆虫所需的时间减少。我们还表明，这两种重组体都能够提高血淋巴中酚氧化酶的活性。小实蝇幼虫在感染的幼虫中蛋白酶的表达导致感染后期内部组织的破坏，这可能是病毒杀死速度增加的原因。杆状病毒及其重组形式构成了化学杀虫剂的可行替代品。含有蛋白酶基因的重组杆状病毒可以被添加到工程杆状病毒的列表中，具有很大的潜力用于害虫综合治理计划。
Baculovirus comprise the largest group of insect viruses most studied worldwide, mainly because they efficiently kill agricutural insect pests. In this study, two recombinant baculoviruses containing the ScathL gene from Sarcophaga peregrina (vSynScathL), and the Keratinase gene from the fungus Aspergillus fumigatus (vSynKerat), were constructed. and their insecticidal properties analysed against Spodoptera frugiperda larvae. Bioassays of third-instar and neonate S. frugiperda larvae with vSynScathL and vSynKerat showed a decrease in the time needed to kill the infected insects when compared to the wild type virus. We have also shown that both recombinants were able to increase phenoloxidase activity in the hemolymph of S. frugiperda larvae. The expression of proteases in infected larvae resulted in destruction of internal tissues late in infection, which could be the reason for the increased viral speed of kill. Baculoviruses and their recombinant forms constitute viable alternatives to chemical insecticides. Recombinant baculoviruses containing protease genes can be added to the list of engineered baculoviruses with great potential to be used in integrated pest management programs.