Satellite cell activation and apoptosis in skeletal muscle from severely burned children
Satellite cell activation and apoptosis in skeletal muscle from severely burned children
复制标题
DOI：
10.1113/jp272520
复制
发表时间：
2016-09-15
影响因子：
5.5
通讯作者：
Finnerty, Celeste C.
中科院分区：
文献类型：
--
作者：
Fry, Christopher S.;Porter, Craig;Finnerty, Celeste C.
严重烧伤会导致严重的骨骼肌萎缩，阻碍恢复。严重烧伤后骨骼肌干细胞（卫星细胞）的活性尚不清楚，可能有助于肌肉的恢复。严重烧伤诱导骨骼肌再生和凋亡。卫星细胞在烧伤后急性发生凋亡和活化，与健康对照组相比，卫星细胞含量净减少。卫星细胞的激活和凋亡可能会影响严重烧伤后瘦组织的恢复，导致烧伤survivors.AbstractSevere烧伤导致严重的骨骼肌萎缩;持续的肌肉损失和虚弱是阻碍烧伤恢复的主要并发症。许多因素导致烧伤后肌肉质量的侵蚀，我们认为受损的肌肉卫星细胞反应是烧伤引起的恶病质的病因学的关键。从肌肉活检。股外侧肌获自12名男性小儿烧伤患者（>30%总体表面积烧伤）和12名年轻健康男性受试者。卫星细胞含量，激活和凋亡，通过免疫组化，肌纤维再生和肌细胞凋亡。胚胎肌球蛋白重链表达和中央成核是骨骼肌再生的指标，烧伤患者的骨骼肌再生指数升高（P
Key points Severe burns result in profound skeletal muscle atrophy that hampers recovery. The activity of skeletal muscle stem cells, satellite cells, acutely following a severe burn is unknown and may contribute to the recovery of lean muscle. Severe burn injury induces skeletal muscle regeneration and myonuclear apoptosis. Satellite cells undergo concurrent apoptosis and activation acutely following a burn, with a net reduction in satellite cell content compared to healthy controls. The activation and apoptosis of satellite cells probably impacts the recovery of lean tissue following a severe burn, contributing to prolonged frailty in burn survivors.AbstractSevere burns result in profound skeletal muscle atrophy; persistent muscle loss and weakness are major complications that hamper recovery from burn injury. Many factors contribute to the erosion of muscle mass following burn trauma and we propose that an impaired muscle satellite cell response is key in the aetiology of burn-induced cachexia. Muscle biopsies from the m. vastus lateralis were obtained from 12 male pediatric burn patients (>30% total body surface area burn) and 12 young, healthy male subjects. Satellite cell content, activation and apoptosis were determined via immunohistochemistry, as were muscle fibre regeneration and myonuclear apoptosis. Embryonic myosin heavy chain expression and central nucleation, indices of skeletal muscle regeneration, were elevated in burn patients (P