Biofilm formation in environmental bacteria is influenced by different macromolecules depending on genus and species
Biofilm formation in environmental bacteria is influenced by different macromolecules depending on genus and species
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DOI：
10.1111/j.1462-2920.2010.02223.x
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发表时间：
2010-09-01
影响因子：
5.1
通讯作者：
Banwart, Steven A.
中科院分区：
文献类型：
--
作者：
Andrews, Johanna S.;Rolfe, Stephen A.;Banwart, Steven A.
从污染土壤和地下水中分离的不同细菌在不同表面性质的模型基质上形成生物膜的情况在多因素筛选中进行了评估。通过结晶紫染料保留和共聚焦激光扫描显微镜（CLSM）观察到不同的附着表型。细胞疏水性的批量测量几乎没有预测能力，以确定是否生物膜将开发疏水或亲水基质。因此，使用CLSM和亲脂性染料尼罗红更详细地检查了来自红球菌属、假单胞菌属和鞘氨醇单胞菌属的表现出不同附着表型的所选细菌对。红球菌属细胞膜与脂质的结合显示出影响这些细胞的附着特性，但这种方法对假单胞菌属和鞘氨醇单胞菌属没有信息。红球菌生物膜的共聚焦拉曼显微光谱证实了脂质在其形成中的重要性，并表明在假单胞菌属和鞘氨醇单胞菌属生物膜中，核酸和蛋白质分别在确定所选菌株的附着表型差异方面很重要。用DNA酶I处理生物膜证实了如假单胞菌所预测的核酸的决定作用。这项工作表明，环境样本中的微生物对不同基质的附着表型存在显着差异，可能涉及多种大分子，并且这些在属之间存在显着差异。CLSM和拉曼光谱的组合区分表型，并可用于确定参与细胞附着到所研究的特定情况下的表面的关键大分子。
P>The formation of biofilms by diverse bacteria isolated from contaminated soil and groundwater on model substrata with different surface properties was assessed in a multifactorial screen. Diverse attachment phenotypes were observed as measured by crystal violet dye retention and confocal laser scanning microscopy (CLSM). Bulk measurements of cell hydrophobicity had little predictive ability in determining whether biofilms would develop on hydrophobic or hydrophilic substrata. Therefore selected pairs of bacteria from the genera Rhodococcus, Pseudomonas and Sphingomonas that exhibited different attachment phenotypes were examined in more detail using CLSM and the lipophilic dye, Nile Red. The association of Rhodococcus sp. cell membranes with lipids was shown to influence the attachment properties of these cells, but this approach was not informative for Pseudomonas and Sphingomonas sp. Confocal Raman Microspectroscopy of Rhodococcus biofilms confirmed the importance of lipids in their formation and indicated that in Pseudomonas and Sphingomonas biofilms, nucleic acids and proteins, respectively, were important in identifying the differences in attachment phenotypes of the selected strains. Treatment of biofilms with DNase I confirmed a determining role for nucleic acids as predicted for Pseudomonas. This work demonstrates that the attachment phenotypes of microbes from environmental samples to different substrata varies markedly, a diverse range of macromolecules may be involved and that these differ significantly between genera. A combination of CLSM and Raman spectroscopy distinguished between phenotypes and could be used to identify the key macromolecules involved in cell attachment to surfaces for the specific cases studied.