Extracellular Vesicle Isolation by a Tangential-Flow Filtration-Based Large-Scale Purification Method.
Extracellular Vesicle Isolation by a Tangential-Flow Filtration-Based Large-Scale Purification Method.
复制标题
通过基于切向流过滤的大规模纯化方法分离细胞外囊泡。
DOI：
10.1007/978-1-0716-3203-1_5
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Dittmer,DirkP
中科院分区：
文献类型：
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作者：
Yuan,Runjie;Zhou,Yijun;Arias,GabrielF;Dittmer,DirkP
Extracellular vesicle (EV) isolation from conditioned cell culture medium has been a challenging topic. It is particularly difficult to obtain pure and intact EVs at a large scale. The commonly used methods such as differential centrifugation, ultracentrifugation, size exclusion chromatography, polyethylene glycol (PEG) precipitation, filtration, and affinity-based purification each have their advantages and limitations. Here, we present a tangential-flow filtration (TFF) based, multi-step purification protocol that combines filtration, PEG precipitation, and Capto Core 700 multimodal chromatography (MMC) to isolate EVs at high purity from large volumes of cell culture conditioned medium. Inserting the TFF step before PEG precipitation removes proteins, which may aggregate in subsequent steps and co-purify with EVs.
