Differences in the immune responses in lambs and kids vaccinated against paratuberculosis, according to the age of vaccination
Differences in the immune responses in lambs and kids vaccinated against paratuberculosis, according to the age of vaccination
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DOI：
10.1016/s0378-1135(00)00332-1
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发表时间：
2000-12-20
影响因子：
3.3
通讯作者：
Marín, JFG
中科院分区：
文献类型：
--
作者：
Corpa, JM;Pérez, V;Marín, JFG
为评价和比较副结核疫苗免疫诱导的外周免疫应答与免疫日龄的关系，分别在15日龄和5月龄对羔羊和山羊羔进行免疫。将热灭活的商业疫苗皮下接种，并分别在羔羊中0、30、90、180、270和360 dpv以及山羊中0、30、90和180 dpv时通过ELISA和IFN-γ测定法测量体液和细胞免疫应答。IFN-γ值在两组之间未显示统计学显著差异，但与未接种对照组相比，在15日龄接种疫苗的动物中，这种细胞因子应答往往更早消失。在5个月时接种疫苗的动物中，抗体应答始终更高且更持久。建议15日龄动物免疫系统成熟程度不完全可能是疫苗接种免疫应答差异的原因。(C)2000 Elsevier Science B. V.保留所有权利。
In order to evaluate and compare the peripheral immune responses induced by the vaccination against paratuberculosis in relation with the age of immunization, two groups of lambs and goat kids were vaccinated at 15 days and 5 months old, respectively. A heat-killed commercial vaccine was inoculated subcutaneously and humoral and cellular immune responses were measured by an ELISA and IFN-gamma assay, respectively, at 0, 30, 90, 180, 270 and 360 dpv in the lambs and 0, 30, 90 and 180 dpv in the caprine. IFN-gamma values did not show statistically significant differences between both groups, but when compared to the unvaccinated controls, this cytokine response tend to disappear earlier in animals vaccinated at 15 days old. The antibody response was always higher and more persistent in animals vaccinated at 5 months. The possibility of the incomplete degree of maturation of the immune system in 15 days old animals as the cause of the differences in the immune response to vaccination is suggested. (C) 2000 Elsevier Science B.V. All rights reserved.