Common Repository of FBS Proteins (cRFP) To Be Added to a Search Database for Mass Spectrometric Analysis of Cell Secretome
Common Repository of FBS Proteins (cRFP) To Be Added to a Search Database for Mass Spectrometric Analysis of Cell Secretome
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DOI：
10.1021/acs.jproteome.9b00475
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发表时间：
2019-10-01
影响因子：
4.4
通讯作者：
Lee, Cheolju
中科院分区：
文献类型：
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作者：
Shin, Jihye;Kwon, Yumi;Lee, Cheolju
我们建议在细胞分泌组的 MS（质谱）原始数据搜索中使用 cRFP（胎牛血清蛋白通用存储库）。 cRFP 是添加到正在使用的参考数据库中的高丰度胎牛血清蛋白的小型补充序列表。使用 cRFP 的目的是防止污染性 FBS 蛋白质被错误识别为参考数据库中的其他蛋白质，就像我们使用 cRAP（通用外来蛋白质存储库）来防止意外或通过不可避免的接触而存在的污染性蛋白质被错误识别为其他蛋白质一样。我们期望它能够广泛应用于实验中，其中蛋白质是从彻底洗涤细胞后的无血清培养基中获得的，或者是从复杂的培养基（如 SILAC）中获得的，或者是直接从细胞外囊泡中获得的。
We propose to use cRFP (common Repository of FBS Proteins) in the MS (mass spectrometry) raw data search of cell secretomes. cRFP is a small supplementary sequence list of highly abundant fetal bovine serum proteins added to the reference database in use. The aim behind using cRFP is to prevent the contaminant FBS proteins from being misidentified as other proteins in the reference database, just as we would use cRAP (common Repository of Adventitious Proteins) to prevent contaminant proteins present either by accident or through unavoidable contacts from being misidentified as other proteins. We expect it to be widely used in experiments where the proteins are obtained from serum-free media after thorough washing of the cells, or from a complex media such as SILAC, or from extracellular vesicles directly.