Protein-heme interactions in hemoglobin from the mollusc Scapharca inaequivalvis: evidence from resonance Raman scattering.
Protein-heme interactions in hemoglobin from the mollusc Scapharca inaequivalvis: evidence from resonance Raman scattering.
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来自软体动物 Scapharca inaequivalvis 的血红蛋白中的蛋白质-血红素相互作用：来自共振拉曼散射的证据。
DOI：
10.1021/bi00076a005
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发表时间：
1993
期刊：
影响因子：
2.9
通讯作者：
Rousseau,DL
中科院分区：
文献类型：
--
作者：
Song,S;Boffi,A;Chiancone,E;Rousseau,DL
材料和方法根据已建立的方法（Chiancone等，1981年）。通过Rossi-Fanelli等人的方法新鲜制备人HbA。（一九五八年）。肌红蛋白（抹香鲸）从Sigma获得，使用前过滤，共振拉曼测量样品在0.1 M磷酸盐缓冲液中制备，pH 7.0，蛋白质浓度为150-250 µ血红素。对于脱氧衍生物的光谱，通过在池中冲洗N2使样品脱氧，并且在测量之前添加少量新鲜连二亚硫酸钠溶液。如Boffi等人所报道的制备对-（氯汞）苯甲酸酯（PMB）修饰的Hbl。（1987年）。通过共振拉曼差分技术（Rousseau，1981）进行拉曼测量。将样品放置在圆柱形池中，所述圆柱形池沿直径具有沿着，使得待比较的样品在池旋转时交替地被激光照射。散射光用Spex单色仪（Spex Industries，梅图陈，NJ）分散，并通过与AT&T个人计算机连接的光电倍增管检测。所有光谱均在室温下测量。对于13 CO [ICON，Marion，NY; 99%13C（原子百分比）]同位素研究，在N2气氛下将脱氧Hbl放置在分裂旋转池的每一侧中，并用橡胶塞密封两个隔室。将13 CO或12 CO直接注入样品中。对于1 S 〇 2 [ICON; 97%180（原子百分比）]测量，将密封在池中的脱气样品用氧同位素在约Iatm的分压下冲洗数次。在共振拉曼测量中使用441.6和413.1 nm的入射激光频率。对于CO衍生物的测量，通过滤光器将激光功率降低至约2 mW以使光解离最小化。
MATERIALS AND METHODSScapharca inaequivalvis Hbl was isolated and purified according to established methods (Chiancone et al., 1981). Human HbA was freshly prepared by the method of Rossi-Fanelli et al.(1958). Myoglobin (sperm whale) was obtained from Sigma and filtered before use.Samples for resonance Raman measurements were prepared in 0.1 M phosphate buffer at pH 7.0 and at a protein concentration of 150-250 µ heme. For the spectra of the deoxy derivative, the sample was deoxygenated by flushing N2 in the cell, and a small amount of fresh sodium dithionite solution was added just before the measurements. p-(Chlo-romercuri) benzoate (PMB)-modified Hbl was prepared as reported by Boffi et al.(1987). The Raman measurements were carried out by a resonance Raman difference technique (Rousseau, 1981). Samples were placed in a cylindrical cell with a partition along the diameter such that the samples to be compared are illuminated alternatively by the laser as the cell is rotated. Scattered light was dispersed with a Spex monochromator (Spex Industries, Metuchen, NJ) and detected by a photomultiplier which was interfaced to an AT&T personal computer. All the spectra were measured at room temperature. For13 CO [ICON, Marion, NY; 99% 13C (atom percent)] isotope studies, deoxygenated Hbl was placed in each side of a split rotating cell under an N2 atmosphere, and both compartments were sealed with rubber stoppers. Either 13CO or 12CO was injected directly into the sample. For the 1S02 [ICON; 97% 180 (atom percent)] measurements, the degassed sample, sealed in the cell, was flushed several times with the oxygen isotope at a partial pressure of about 1 atm. Incident laser frequencies of 441.6 and 413.1 nm were used in the resonance Raman measurements. For measurements of the CO derivatives, the laser power was decreased to about 2 mW by an optical filter to minimize photodissociation.
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蚶二聚血红蛋白的 2.4-A 晶体结构。
影响因子：
4.8
作者：
W. Royer;W. Hendrickson;E. Chiancone
通讯作者：
E. Chiancone
I 类组织相容性分子的细胞内转运。
DOI：
--
发表时间：
1988
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Williams,DB;Borriello,F;Zeff,RA;Nathenson,SG
通讯作者：
Nathenson,SG
来自软体动物 Scapharca inaequivalvis 的协同同二聚血红蛋白的核磁共振研究。
DOI：
10.1111/j.1432-1033.1989.tb14989.x
发表时间：
1989
期刊：
European journal of biochemistry
影响因子：
--
作者：
McGourty,JL;LaMar,GN;Smith,KM;Ascoli,F;Chiancone,E;Pandey,RK;Singh,JP
通讯作者：
Singh,JP
DOI：
--
发表时间：
1982
期刊：
Biochimica et Biophysica Acta
影响因子：
--
作者：
D. Verzili;R. Santucci;M. Ikeda;E. Chiancone;F. Ascoli;T. Yonetani;E. Antonini
通讯作者：
E. Antonini
血红蛋白四级结构转变的共振拉曼散射研究。
DOI：
10.1146/annurev.bb.12.060183.002041
发表时间：
1983
期刊：
Annual review of biophysics and bioengineering
影响因子：
--
作者：
D. Rousseau;M. Ondrias
通讯作者：
M. Ondrias