Analysis of Campylobacter jejuni infection in the gnotobiotic piglet and genome-wide identification of bacterial factors required for infection.
Analysis of Campylobacter jejuni infection in the gnotobiotic piglet and genome-wide identification of bacterial factors required for infection.
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DOI：
10.1038/srep44283
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发表时间：
2017-03-10
影响因子：
4.6
通讯作者：
Everest P
中科院分区：
文献类型：
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作者：
de Vries SP;Linn A;Macleod K;MacCallum A;Hardy SP;Douce G;Watson E;Dagleish MP;Thompson H;Stevenson A;Kennedy D;Baig A;Coward C;Maskell DJ;Smith DG;Grant AJ;Everest P
为了研究空肠弯曲菌如何引起人类肠道疾病的临床症状，使用相关的动物模型是必不可少的。这种模型应在宿主生理学、疾病发作前的潜伏期、临床体征和可比的疾病结局方面密切模拟人类疾病。本研究利用仔猪模型研究了克雷伯氏菌致病性的决定因素。空肠。在该模型中，C.空肠成功地建立了感染，仔猪出现了体温升高伴水样腹泻，这是由肠内上皮细胞渗漏和胆汁重吸收减少引起的。此外，我们评估了C。利用转座子（Tn）突变体文库筛选猪胃肠道感染所需的空肠基因。共鉴定了123个基因，其中Tn突变体表现出弱毒仔猪感染。我们的筛选突出了运动性和趋化性以及中枢代谢的关键作用。此外，14个基因的Tn突变体显示增强仔猪感染。本研究对C.在宿主生理学和促成细菌因素方面，空肠疾病。
To investigate how Campylobacter jejuni causes the clinical symptoms of diarrhoeal disease in humans, use of a relevant animal model is essential. Such a model should mimic the human disease closely in terms of host physiology, incubation period before onset of disease, clinical signs and a comparable outcome of disease. In this study, we used a gnotobiotic piglet model to study determinants of pathogenicity of C. jejuni. In this model, C. jejuni successfully established infection and piglets developed an increased temperature with watery diarrhoea, which was caused by a leaky epithelium and reduced bile re-absorption in the intestines. Further, we assessed the C. jejuni genes required for infection of the porcine gastrointestinal tract utilising a transposon (Tn) mutant library screen. A total of 123 genes of which Tn mutants showed attenuated piglet infection were identified. Our screen highlighted a crucial role for motility and chemotaxis, as well as central metabolism. In addition, Tn mutants of 14 genes displayed enhanced piglet infection. This study gives a unique insight into the mechanisms of C. jejuni disease in terms of host physiology and contributing bacterial factors.