IPS-1, an adaptor triggering RIG-I- and Mda5-mediated type I interferon induction
IPS-1, an adaptor triggering RIG-I- and Mda5-mediated type I interferon induction
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DOI：
10.1038/ni1243
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发表时间：
2005-10-01
影响因子：
30.5
通讯作者：
Akira, S
中科院分区：
文献类型：
--
作者：
Kawai, T;Takahashi, K;Akira, S
I型干扰素是抗病毒反应的中心介体。使用干扰素诱导剂的高通量功能筛选，我们在这里确定了一个分子，我们称之为干扰素β启动子刺激器1（IPS-1）。 IPS-1诱导I型干扰素和干扰素诱导的基因的过表达，通过激活IRF3，IRF7和NF-KAPPA B转录因子。 IPS-1介导的干扰素诱导需要TBK1和IKKI蛋白激酶。 IPS-1包含一种N末端卡样结构，介导与RIG-I和MDA5的卡相互作用，后者是感染病毒感染的细胞质RNA解旋酶。通过小干扰RNA对IPS-1的“敲低”通过病毒感染阻断了干扰素诱导。因此，IPS-1是参与RIGI-I和MDA5介导的抗病毒免疫反应的适配器。
Type I interferons are central mediators for antiviral responses. Using high-throughput functional screening of interferon inducers, we have identified here a molecule we call interferon-beta promoter stimulator 1 (IPS-1). Overexpression of IPS-1 induced type I interferon and interferon-inducible genes through activation of IRF3, IRF7 and NF-kappa B transcription factors. TBK1 and IKKi protein kinases were required for the IPS-1-mediated interferon induction. IPS-1 contained an N-terminal CARD-like structure that mediated interaction with the CARD of RIG-I and Mda5, which are cytoplasmic RNA helicases that sense viral infection. 'Knockdown' of IPS-1 by small interfering RNA blocked interferon induction by virus infection. Thus, IPS-1 is an adaptor involved in RIG-I-and Mda5-mediated antiviral immune responses.