Comparing Transient Oligonucleotide Hybridization Kinetics Using DNA-PAINT and Optoplasmonic Single-Molecule Sensing on Gold Nanorods
Comparing Transient Oligonucleotide Hybridization Kinetics Using DNA-PAINT and Optoplasmonic Single-Molecule Sensing on Gold Nanorods
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DOI：
10.1021/acsphotonics.1c01179
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发表时间：
2021-09-08
期刊：
影响因子：
7
通讯作者：
Vollmer, Frank
中科院分区：
文献类型：
--
作者：
Eerqing, Narima;Subramanian, Sivaraman;Vollmer, Frank
我们报告了两个单分子传感的光子技术：荧光纳米显微镜和光等离子体传感的比较。作为测试系统，具有和不具有荧光标记的寡核苷酸与连接到金纳米棒的互补“对接”链瞬时杂交。比较所测量的单分子动力学有助于检查荧光标记的影响以及由不同的传感几何形状引起的因素。我们的研究结果表明，DNA解离没有显着改变的荧光标记和DNA协会的几何因素的影响，在这两种技术。这些发现为以互补的方式利用等离子体传感和荧光纳米显微镜打开了大门，这将有助于构建更强大的传感器，并揭示影响单分子行为的复杂效应。
We report a comparison of two photonic techniques for single-molecule sensing: fluorescence nanoscopy and optoplasmonic sensing. As the test system, oligonucleotides with and without fluorescent labels are transiently hybridized to complementary "docking" strands attached to gold nanorods. Comparing the measured single-molecule kinetics helps to examine the influence of the fluorescent labels as well as factors arising from different sensing geometries. Our results demonstrate that DNA dissociation is not significantly altered by the fluorescent labels and that DNA association is affected by geometric factors in the two techniques. These findings open the door to exploiting plasmonic sensing and fluorescence nanoscopy in a complementary fashion, which will aid in building more powerful sensors and uncovering the intricate effects that influence the behavior of single molecules.