EVIDENCE FOR GLUTATHIONE-PEROXIDASE ACTIVITIES IN CULTURED PLANT-CELLS
EVIDENCE FOR GLUTATHIONE-PEROXIDASE ACTIVITIES IN CULTURED PLANT-CELLS
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DOI：
10.1016/0168-9452(85)90025-1
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发表时间：
1985-12-02
期刊：
影响因子：
5.2
通讯作者：
FALL, R
中科院分区：
文献类型：
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作者：
DROTAR, A;PHELPS, P;FALL, R
菠菜[Spinacia oleracea]、玉米[Zea mays]和西卡莫尔[Acer pseudoplatanus]的培养植物细胞以及浮萍植物的提取物含有可检测的谷胱甘肽过氧化物酶活性，使用过氧化氢酶或叔丁基过氧化氢作为底物。使用从培养的玉米细胞提取物，谷胱甘肽过氧化物酶活性的两个峰可以通过凝胶过滤和离子交换色谱的组合来解决。一个峰沿着谷胱甘肽转移酶活性洗脱;第二个峰与谷胱甘肽转移酶和抗坏血酸过氧化物酶不同，对过氧化氢和有机氢过氧化物均具有活性。在高等植物细胞中，似乎至少存在两种谷胱甘肽过氧化物酶。
Extracts from cultured plant cells of spinach [Spinacia oleracea], maize [Zea mays] and sycamore [Acer pseudoplatanus] and from Lemna plants contain detectable glutathione peroxidase activity, using either hydrogen peroxidase or t-butyl hydroperoxide as substrates. Using extracts from cultured maize cells, two peaks of glutathione peroxidase activity could be resolved by a combination of gel filtration and ion exchange chromatography. One peak was eluted along with glutathione transferase activity; the second was distinct from both glutathione transferase and ascorbic acid peroxidase, and was active with both hydrogen peroxide and organic hydroperoxides. It seems likely that at least two enzymes with glutathione peroxidase exist in higher plant cells.