The complete genome sequence of the murine respiratory pathogen Mycoplasma pulmonis
The complete genome sequence of the murine respiratory pathogen Mycoplasma pulmonis
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DOI：
10.1093/nar/29.10.2145
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发表时间：
2001-05-15
影响因子：
14.9
通讯作者：
Blanchard, A
中科院分区：
文献类型：
--
作者：
Chambaud, I;Heilig, R;Blanchard, A
肺支原体是一种无壁真细菌，属于毛利门（俗称支原体），并负责小鼠呼吸道疾病。菌株UAB CTIP基因组由一条963 879 bp的单圆形染色体组成，G + C含量为26.6 mol%，除解脲支原体外，是报道的细菌中最低的。该基因组包含782个假设编码序列（CDSs），占其长度的91.4%，其中486个CDSs具有功能，92个CDSs与假设蛋白质的基因序列相匹配，其余204个CDSs没有显著的数据库匹配。基因组包含一组rRNA基因和29个tRNAs基因。通过序列分析和G + C偏态法确定了复制起始位点。重复核苷酸片段内的序列多态性产生相位可变的蛋白抗原，而重组酶基因可能催化主要肺分枝杆菌表面抗原的位点特异性DNA倒位。此外，一个溶血素，分泌核酸酶和一个糖蛋白酶是预测的毒力因子。令人惊讶的是，先前报道的对自我复制的最小细胞至关重要的几个基因在肺支原体基因组中缺失，尽管这个基因组比迄今为止完全测序的其他支原体基因组大。
Mycoplasma pulmonis is a wall-less eubacterium belonging to the Mollicutes (trivial name, mycoplasmas) and responsible for murine respiratory diseases. The genome of strain UAB CTIP is composed of a single circular 963 879 bp chromosome with a G + C content of 26.6 mol%, i.e. the lowest reported among bacteria, Ureaplasma urealyticum apart. This genome contains 782 putative coding sequences (CDSs) covering 91.4% of its length and a function could be assigned to 486 CDSs whilst 92 matched the gene sequences of hypothetical proteins, leaving 204 CDSs without significant database match. The genome contains a single set of rRNA genes and only 29 tRNAs genes. The replication origin oriC was localized by sequence analysis and by using the G + C skew method. Sequence polymorphisms within stretches of repeated nucleotides generate phase-variable protein antigens whilst a recombinase gene is likely to catalyse the site-specific DNA inversions in major M.pulmonis surface antigens. Furthermore, a hemolysin, secreted nucleases and a glycoprotease are predicted virulence factors. Surprisingly, several of the genes previously reported to be essential for a self-replicating minimal cell are missing in the M.pulmonis genome although this one is larger than the other mycoplasma genomes fully sequenced until now.