CD40 ligation in vivo induces bystander proliferation of memory phenotype CD8 T cells
CD40 ligation in vivo induces bystander proliferation of memory phenotype CD8 T cells
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DOI：
10.4049/jimmunol.172.8.4804
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发表时间：
2004-04-15
影响因子：
4.4
通讯作者：
Pircher, H
中科院分区：
文献类型：
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作者：
Koschella, M;Voehringer, D;Pircher, H
已显示将激动性抗CD 40 Ab注射到小鼠中会放大对免疫原性差的化合物的弱CD 8 T细胞应答，并将T细胞耐受性转化为T细胞引发。在这项研究中，我们证明，抗CD 40治疗的C57 BL/6小鼠，没有Ag交付，导致记忆表型CD 4和CD 8 T细胞的数量显着增加。使用CD 40缺陷型宿主的连续转移实验进一步揭示了由系统性CD 40信号传导诱导的记忆T细胞的增殖应答依赖于宿主APC的CD 40表达。体内CD 40连接以部分IL-15依赖性方式诱导记忆表型和真正病毒特异性记忆CD 8 T细胞的剧烈细胞分裂。然而，只有记忆表型，而不是Ag-经验的记忆CD 8 T细胞在体内抗CD 40治疗后细胞数量增加。总之，我们的数据表明，通过CD 40激活APC诱导记忆表型T细胞的显着旁观者增殖。此外，我们证明了真正的Ag经历的记忆CD 8 T细胞与记忆表型CD 8 T细胞对抗CD 40诱导的信号的反应不同。
Injection of agonistic anti-CD40 Abs into mice has been shown to amplify weak CD8 T cell responses to poorly immunogenic compounds and to convert T cell tolerance to T cell priming. In this study we demonstrate that anti-CD40 treatment of C57BL/6 mice, without Ag delivery, led to a marked increase in the number of memory phenotype CD4 and CD8 T cells. Adoptive transfer experiments using CD40-deficient hosts further revealed that the proliferative response of memory T cells, induced by systemic CD40 signaling, was dependent on CD40 expression of host APCs. CD40 ligation in vivo induced vigorous cell division of both memory phenotype and bona fide virus-specific memory CD8 T cells in a partially IL-15-dependent manner. However, only memory phenotype, but not Ag-experienced memory CD8 T cells increased in cell number after anti-CD40 treatment in vivo. Taken together our data show that activation of APC via CD40 induces a marked bystander proliferation of memory phenotype T cells. In addition, we demonstrate that bona fide Ag-experienced memory CD8 T cells respond differently to anti-CD40-induced signals than memory phenotype CD8 T cells.