The Human Proteome Project: Current State and Future Direction
The Human Proteome Project: Current State and Future Direction
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DOI：
10.1074/mcp.m111.009993
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发表时间：
2011-07-01
影响因子：
7
通讯作者：
Omenn, Gilbert S.
中科院分区：
文献类型：
--
作者：
Legrain, Pierre;Aebersold, Ruedi;Omenn, Gilbert S.
在人类基因组计划成功完成后，人类蛋白质组组织最近正式启动了全球人类蛋白质组计划（HPP），旨在绘制整个人类蛋白质组。由于估计的20，300个蛋白质编码基因中约有30%缺乏蛋白质水平的证据，因此有必要在蛋白质丰度、分布、亚细胞定位、与其他生物分子的相互作用以及特定时间点的功能方面进行系统的全球努力以实现这一目标。作为一般的实验策略，HPP研究小组将使用HPP的三个工作支柱：质谱，抗体捕获和生物信息学工具和知识库。HPP参与者将利用正在进行的人类蛋白质组组织倡议的输出和交叉分析以及以染色体为中心的蛋白质作图策略（称为C-HPP），许多国家团队目前正在参与该策略。此外，许多生物驱动和以疾病为导向的项目将得到HPP的刺激和促进。通过对HPP的适当管理，及时规划将提供蛋白质部件清单，试剂和蛋白质研究和分析工具，以及个性化医疗的更坚实基础。人类蛋白质组组织敦促每个国家研究资助机构和科学界确定他们的首选途径，以参与资助者和研究人员组成的HPP联盟中这个非常有前途的项目的各个方面。Molecular & Cellular Proteomics 10：10.1074/mcp. M111.009993，1 - 5，2011.
After the successful completion of the Human Genome Project, the Human Proteome Organization has recently officially launched a global Human Proteome Project (HPP), which is designed to map the entire human protein set. Given the lack of protein-level evidence for about 30% of the estimated 20,300 protein-coding genes, a systematic global effort will be necessary to achieve this goal with respect to protein abundance, distribution, subcellular localization, interaction with other biomolecules, and functions at specific time points. As a general experimental strategy, HPP research groups will use the three working pillars for HPP: mass spectrometry, antibody capture, and bioinformatics tools and knowledge bases. The HPP participants will take advantage of the output and cross-analyses from the ongoing Human Proteome Organization initiatives and a chromosome-centric protein mapping strategy, termed C-HPP, with which many national teams are currently engaged. In addition, numerous biologically driven and disease-oriented projects will be stimulated and facilitated by the HPP. Timely planning with proper governance of HPP will deliver a protein parts list, reagents, and tools for protein studies and analyses, and a stronger basis for personalized medicine. The Human Proteome Organization urges each national research funding agency and the scientific community at large to identify their preferred pathways to participate in aspects of this highly promising project in a HPP consortium of funders and investigators. Molecular & Cellular Proteomics 10: 10.1074/mcp.M111.009993, 1-5, 2011.