Involvement of the LSPR spectral overlap for energy transfer between a dye and Au nanoparticle.
Involvement of the LSPR spectral overlap for energy transfer between a dye and Au nanoparticle.
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DOI：
10.1021/ja1022128
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发表时间：
2010-06
影响因子：
15
通讯作者：
Manita Singh;G. Strouse
中科院分区：
文献类型：
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作者：
Manita Singh;G. Strouse
纳米金属表面能量转移（NSET）是一种分子标尺技术，已被用于光学探测生物分子结构中的长距离。我们研究了供体染料的有用光谱范围以及局部表面等离子体共振（LSPR）和供体光致发光（520-780 nm）之间重叠的重要性，并提供了从染料到2 nm Au NP（金纳米颗粒）的NSET过程的R（0）值的全面研究。距离依赖性淬灭结果提供了实验证据，即效率曲线斜率、R（0）值和淬灭距离最好建模为表面介导的NSET过程，类似于Casson-Lang和Chance-Prock-Silbey（CPS）的预测。结果表明，LSPR在所观察到的激发态施主在纳米金属表面的猝灭中起着非常重要的作用，并且与NSET模型的相关性允许在生物物理工具箱内应用必要的生物物理常数的编译。
Nanometal surface energy transfer (NSET) is a molecular ruler technique that has been utilized to optically probe long distances in biomolecular structures. We investigate the useful spectral range of donor dyes and the importance of overlap between the localized surface plasmon resonance (LSPR) and the donor photoluminescence (520-780 nm) and provide a comprehensive study of the R(0) values for the NSET processes from dyes to 2 nm Au NP (gold nanoparticle). The distance-dependent quenching results provide experimental evidence that the efficiency curve slope, R(0) value, and distance of quenching is best modeled as a surface-mediated NSET process analogous to the predictions of Persson-Lang and Chance-Prock-Silbey (CPS). The results show that the LSPR plays a very important role in the observed quenching of excited-state donors at the surface of the nanometal, and the correlation to the NSET model allows a compilation of the necessary biophysical constants for application within the toolbox of biophysics.