Induction of apoptosis by rhapontin having stilbene moiety, a component of rhubarb (Rheum officinale Baillon) in human stomach cancer KATO III cells.
Induction of apoptosis by rhapontin having stilbene moiety, a component of rhubarb (Rheum officinale Baillon) in human stomach cancer KATO III cells.
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DOI：
10.3892/or.18.2.347
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发表时间：
2007-08
期刊：
影响因子：
4.2
通讯作者：
H. Hibasami;Keiji Takagi;T. Ishii;M. Tsujikawa;N. Imai;Ikumi Honda
中科院分区：
文献类型：
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作者：
H. Hibasami;Keiji Takagi;T. Ishii;M. Tsujikawa;N. Imai;Ikumi Honda
本文研究了土大黄素对人胃癌KATO Ⅲ细胞增殖和DNA的影响。在KATO III细胞中观察到土大黄素的生长抑制和凋亡诱导。在用土大黄素处理的KATO III细胞中观察到显示凋亡小体的形态学变化。在KATO III细胞中，观察到土大黄素对DNA的片段化为寡核小体大小的片段，这是细胞凋亡的特征，具有浓度和时间依赖性。抗氧化剂N-乙酰-L-半胱氨酸可抑制土大黄素引起的DNA断裂。另一方面，发现具有芪部分的白藜芦醇以及土大黄素诱导KATO III细胞的凋亡。因此，认为分子中的二苯乙烯部分对于诱导细胞凋亡是必需的。本研究的数据表明，通过Rhapontin的抑制KATO III细胞生长的结果从诱导细胞凋亡的化合物，和活性氧参与诱导细胞凋亡引起的Rhapontin在KATO III细胞。
We have investigated the effects of rhapontin on proliferation and DNA of human stomach cancer KATO III cells. Growth inhibition and induction of apoptosis by rhapontin were observed in the KATO III cells. Morphological change showing apoptotic bodies was observed in the KATO III cells treated with rhapontin. The fragmentation of DNA by rhapontin to oligonucleosomal-sized fragments that is a characteristic of apoptosis was observed to be concentration- and time-dependent in the KATO III cells. N-acetyl-L-cysteine, an antioxidant, suppressed the DNA fragmentation caused by rhapontin. On the other hand, it was found that resveratrol having stilbene moiety as well as rhapontin induced apoptosis in the KATO III cells. So, it is considered that stilbene moiety in the molecule is essential for the induction of apoptosis. The data of the present study show that the suppression of KATO III cell-growth by rhapontin results from the induction of apoptosis by the compound, and that active oxygen is involved in the inductions of apoptosis caused by rhapontin in the KATO III cells.