RNA EDITING OF WHEAT MITOCHONDRIAL ATP SYNTHASE SUBUNIT-9 - DIRECT PROTEIN AND CDNA SEQUENCING
RNA EDITING OF WHEAT MITOCHONDRIAL ATP SYNTHASE SUBUNIT-9 - DIRECT PROTEIN AND CDNA SEQUENCING
复制标题
DOI：
10.1105/tpc.2.12.1283
复制
发表时间：
1990-12-01
期刊：
影响因子：
11.6
通讯作者：
ARAYA, A
中科院分区：
文献类型：
--
作者：
BEGU, D;GRAVES, PV;ARAYA, A
通过cDNA和蛋白质序列分析研究了小麦线粒体ATP合成酶9亚基的RNA编辑。 大多数测序的cDNA克隆（95%）显示，通过C到U转换的编辑发生在编码区的8个位置。 因此，与从该基因预测的序列相比，蛋白质中有5个氨基酸发生了变化。 两个编辑的密码子没有变化（沉默编辑）。 其中一个C到U的转换通过将精氨酸密码子CGA修饰为UGA产生终止密码子。 因此，产生的蛋白质比从基因组序列推导的蛋白质短6个氨基酸。 蛋白质序列和氨基酸组成分析证实了cDNA测序的结果，并表明编辑的atp9 mRNA的主要形式是翻译。
RNA editing of subunit 9 of the wheat mitochondrial ATP synthase has been studied by cDNA and protein sequence analysis. Most of the cDNA clones sequenced (95%) showed that editing by C-to-U transitions occurred at eight positions in the coding region. Consequently, 5 amino acids were changed in the protein when compared with the sequence predicted from the gene. Two edited codons gave no changes (silent editing). One of the C-to-U transitions generated a stop codon by modifying the arginine codon CGA to UGA. Thus, the protein produced is 6 amino acids shorter than that deduced from the genomic sequence. Minor forms of cDNA with partial or overedited sequences were also found. Protein sequence and amino acid composition analyses confirmed the results obtained by cDNA sequencing and showed that the major form of edited atp9 mRNA is translated.