Carbon nanotubes in macrophages: Imaging and chemical analysis by X-ray fluorescence microscopy
Carbon nanotubes in macrophages: Imaging and chemical analysis by X-ray fluorescence microscopy
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DOI：
10.1021/nl800914m
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发表时间：
2008-09-01
期刊：
影响因子：
10.8
通讯作者：
Launoist, Pascale
中科院分区：
文献类型：
--
作者：
Bussy, Cyrill;Cambedouzou, Julien;Launoist, Pascale
首次应用X射线荧光显微镜（mu XRF）研究暴露于未纯化和纯化的单壁（SW）和多壁（MW）碳纳米管（CNT）的巨噬细胞。研究化学元素分布允许人们（I）在细胞内成像纳米管定位和（ii）检测CNT内化后细胞的化学修饰。检测到过量的钙暴露于未纯化的单壁碳纳米管和多壁碳纳米管和相关的毒理学试验进行了讨论。
X-ray fluorescence microscopy (mu XRF) is applied for the first time to study macrophages exposed to unpurified and purified single-walled (SW) and multiwalled (MW) carbon nanotubes (CNT). Investigating chemical elemental distributions allows one to (I) image nanotube localization within a cell and (ii) detect chemical modification of the cell after CNT internalization. An excess of calcium is detected for cells exposed to unpurified SWCNT and MWCNT and related toxicological assays are discussed.