Circadian organization of thirteen liver and six brain enzymes of the mouse.
Circadian organization of thirteen liver and six brain enzymes of the mouse.
复制标题
小鼠十三种肝脏和六种脑酶的昼夜节律组织。
DOI：
10.1002/aja.1001620302
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Casciano,DA
中科院分区：
文献类型：
--
作者：
North,C;Feuers,RJ;Scheving,LE;Pauly,JE;Tsai,TH;Casciano,DA
在7-12周龄CD 2F 1雄性小鼠中研究了13种肝脏酶和6种脑酶的活性，这些小鼠自由采食，并标准化为12小时光照（0600-1800）与12小时黑暗（1800-0600）交替（LD 12：12）或反向明暗循环（黑暗0600-1800;光照1800-0600）（DL 12：12）。在两个不同的日期进行了三项单独的研究;在每项实验中，每隔3小时处死一个亚组的14只动物。对肝脏进行以下分析：在一些实施方案中，所述酶包括但不限于异柠檬酸脱氢酶、谷氨酸脱氢酶、乳酸脱氢酶、醇脱氢酶、谷胱甘肽还原酶、乙醛酸还原酶、L-丙氨酸氨基转移酶、谷氨酸-乙酸转氨酶、丙酮酸脱羧酶、果糖-1-磷酸醛缩酶、果糖二磷酸醛缩酶、果糖1，6-二磷酸酶和脂肪酸合成酶。对大脑进行磷酸葡萄糖异构酶、三磷酸腺苷酶、肌酸磷酸激酶、丙酮酸激酶、腺苷酸激酶和苹果酸脱氢酶的检测，所有19种酶在至少一个实验中表现出明显的昼夜节律。此外，通过最小二乘法，每个节律变量显示出与24小时余弦（正弦）曲线的统计学显著拟合。一般来说，分析的肝酶的峰值活动与黑暗周期的开始和动物活动的开始有关，而脑酶组的峰值活动发生在动物休息时间的开始，接近光周期的开始。在将环境明暗循环逆转180°后，每种节律的相位可以在两周内逆转。
The activities of 13 liver and 6 brain enzymes were studied in 7–12 week old CD2F1male mice that had been fed ad libitum and standardized either to 12 hours of light (0600–1800) alternating with 12 hours of darkness (1800–0600) (LD 12:12), or to a reversed light‐dark cycle (darkness 0600–1800; light 1800–0600) (DL 12:12). Three separate studies were performed on two different days; in each experiment, subgroups of 14 animals were sacrificed at 3‐hour intervals. Livers were assayed for: isocitrate dehydrogenase, glutamate dehydrogenase, lactate dehydrogenase, alcohol dehydrogenase, glutathione reductase, glyoxylate reductase, L‐alanine aminotransferase, glutamate oxalacetate transaminase, pyruvate decarboxylase, fructose‐1‐phosphate aldolase, fructose diphosphate aldolase, fructose 1,6‐diphosphatase, and fatty acid synthetase. Brains were assayed for phosphoglucose isomerase, adenosine triphosphatase, creatine phosphokinase, pyruvate kinase, adenylate kinase, and malate dehydrogenase.All 19 enzymes demonstrated a prominent circadian rhythm in at least one experiment. Moreover, each rhythmic variable showed a statistically significant fit to a 24‐hour cosine(sine) curve by the method of least squares. In general, peak activities of the liver enzymes analyzed were associated with the beginning of the dark cycle and initiation of the animal's activity, while the group of brain enzymes had peak activities which occurred at the beginning of the animals' rest span and were near the beginning of the light cycle. The phasing of each of the rhythms could be reversed within a two‐week span after reversing the environmental light‐dark cycle 180°.