Dog inactive renin: biochemical characterization and secretion into renal plasma and lymph.
Dog inactive renin: biochemical characterization and secretion into renal plasma and lymph.
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狗失活肾素：生化特征和分泌到肾血浆和淋巴液中。
DOI：
10.1152/ajpendo.1986.250.1.e55
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Dunckel,P
中科院分区：
文献类型：
--
作者：
Dzau,VJ;Wilcox,CS;Sands,K;Dunckel,P
有人认为，狗是研究非活性肾素（IR）的有用模型。然而，犬血浆中胰蛋白酶激活的血管紧张素形成活性的性质和来源尚未完全确定。我们使用肾素特异性抗体、免疫亲和和阿菲格尔蓝色谱来表征狗的IR。激活的肾素在生化和免疫特性上与肾素和血浆活性肾素相似。肾淋巴、动脉、静脉血浆及肾提取物均检测到IR。在基础状态下，有明显的肾静脉-动脉IR梯度，表明肾脏分泌。此外，肾淋巴中的IR浓度比血浆高6倍。我们的数据为肾脏IR分泌的两种可能途径提供了证据，并支持了狗是研究IR分泌和调节的良好模型的论点。
It has been suggested that the dog is a useful model for studies of inactive renin (IR). However, the nature and origin of the trypsin-activated angiotensin-forming activity in dog plasma have not been fully defined. We characterized dog IR using renin-specific antibody, immunoaffinity, and Affigel blue chromatography. Activated IR resembles renal and plasma active renin in biochemical and immunological properties. IR was detected in renal lymph, arterial and venous plasma, as well as renal extracts. At basal state, there was a significant renal venous-arterial gradient of IR indicating secretion from the kidney. Furthermore, a sixfold higher concentration of IR was demonstrated in renal lymph than in plasma. Our data provides evidence for two possible routes of IR secretion from the kidney and supports the contention that the dog is a good model for studies of IR secretion and regulation.