PLANT-VIRUS EXPRESSING HYBRID COAT PROTEIN WITH ADDED MURINE EPITOPE ELICITS AUTOANTIBODY RESPONSE
PLANT-VIRUS EXPRESSING HYBRID COAT PROTEIN WITH ADDED MURINE EPITOPE ELICITS AUTOANTIBODY RESPONSE
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DOI：
10.1016/0264-410x(95)00075-c
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发表时间：
1995-08-01
期刊：
影响因子：
5.5
通讯作者：
HEIN, MB
中科院分区：
文献类型：
--
作者：
FITCHEN, J;BEACHY, RN;HEIN, MB
使用修饰的烟草花叶病毒（TMV）指导含有鼠透明质酸ZP 3蛋白的13个氨基酸序列的TMV外壳蛋白杂合体的合成。发现该杂合蛋白是稳健的，并且在接种的植物中积累至高浓度。含有杂合外壳蛋白的病毒样颗粒可以容易地从感染的植物组织中纯化。以这些病毒样颗粒为抗原免疫C57 BL/6 J和BALB/cBy小鼠，可产生识别ZP 3抗原表位的血清抗体，这些抗体可识别合成的ZP 3肽和真实的鼠ZP 3糖蛋白。在雌性小鼠体内将抗体募集至透明质酸。
A modified tobacco mosaic virus (TMV) was used to direct the synthesis of TMV coat protein hybrids containing a 13 amino acid sequence of the murine zona pellucida ZP3 protein. This hybrid protein was found to be robust and to accumulate to high concentrations in inoculated plants, Virus-like particles containing the hybrid coat protein could be readily purified from infected plant tissue. Parenteral immunization with these virus-like particles as antigen resulted in serum antibody recognizing ZP3 epitope in C57BL/6J and BALB/cBy mice, The antibodies recognized synthetic ZP3 peptide and the authentic murine ZP3 glycoprotein. Antibodies were recruited in vivo to the zona pellucida in female mice.