The two-hybrid system to detect protein-protein interactions
The two-hybrid system to detect protein-protein interactions
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DOI：
10.1006/meth.1993.1016
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发表时间：
1993-01-01
期刊：
影响因子：
--
通讯作者：
Fields, Stanley
中科院分区：
文献类型：
--
作者：
Fields, Stanley
The two-hybrid system is a yeast-based genetic assay for detecting protein-protein interactions. In this method, genes encoding two hybrid proteins are constructed: one hybrid consists of the DNA-binding domain of a transcriptional activator fused to some protein "X"; the other consists of the transcriptional activation domain fused to some protein "Y". Interaction between proteins X and Y reconstitutes the function of the transcriptional activator and leads to transcription of a reporter gene containing sites for the DNA-binding domain. This system has been used successfully to analyze interactions of nuclear, cytoplasmic, mitochondrial, and membrane-associated proteins from a variety of organisms. Applications of this system include the following: (i) testing for interaction between known proteins whose genes are available; (ii) delineating the domains of two proteins involved in an interaction; (iii) identifying specific residues of a protein important for interaction with another protein; and (iv) screening libraries of activation domain hybrids to identify proteins that bind to some protein of interest.
