A genome-wide survey and systematic RNAi-based characterization of helicase-like genes in Caenorhabditis elegans
A genome-wide survey and systematic RNAi-based characterization of helicase-like genes in Caenorhabditis elegans
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DOI：
10.1093/dnares/dsm016
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发表时间：
2007-08-31
期刊：
影响因子：
4.1
通讯作者：
Hanaoka, Fumio
中科院分区：
文献类型：
--
作者：
Eki, Toshihiko;Ishihara, Takeshi;Hanaoka, Fumio
解旋酶样蛋白在核酸和染色质介导的反应中起着至关重要的作用。在这项研究中，我们确定了134个解旋酶样蛋白在线虫线虫和分类的蛋白质到10个已知的亚家族和一组孤儿基因的基础上的序列相似性。我们的特点是在RNA干扰（RNAi）处理的动物解旋酶家族成员的功能丧失表型，使用RNAi喂养方法，并发现了几个以前未报道的表型。51（39.5%）的129个基因的测试显示发育或生长缺陷的表型，这些基因中的许多是推定的线虫同源的必需基因在单细胞真核生物，芽殖酵母，这表明这些必需蛋白在这两个物种的保护。这些物种之间的比较分析确定了进化分歧线虫蛋白以及保守的家庭成员。对线虫基因的染色体定位显示了10对可能的重复基因和C. elegans特异性SNF 2样基因和Helitrons。转录谱数据分析显示，主要是卵子和生殖细胞丰富的许多解旋酶样基因的表达。最后，我们确定了D2005.5（drh-3）基因在一个基于RNAi的筛选参与耐X射线照射的基因。对DRH-3的分析将阐明其保护C.优雅的
Helicase-like proteins play a crucial role in nucleic acid- and chromatin-mediated reactions. In this study, we identified 134 helicase-like proteins in the nematode Caenorhabditis elegans and classified the proteins into 10 known subfamilies and a group of orphan genes on the basis of sequence similarity. We characterized loss-of-function phenotypes in RNA interference (RNAi)-treated animals for helicase family members, using the RNAi feeding method, and found several previously unreported phenotypes. Fifty-one (39.5%) of 129 genes tested showed development- or growth-defect phenotypes, and many of these genes were putative nematode homologs of essential genes in a unicellular eukaryote, budding yeast, suggesting conservation of these essential proteins in both species. Comparative analyses between these species identified evolutionarily diverged nematode proteins as well as conserved family members. Chromosome mapping of the nematode genes revealed 10 pairs of putative duplicated genes and clusters of C. elegans-specific SNF2-like genes and Helitrons. Analyses of transcriptional profile data revealed a predominantly oogenesis- and germline-enriched expression of many helicase-like genes. Finally, we identified the D2005.5(drh-3) gene in an RNAi-based screen for genes involved in resistance to X-ray irradiation. Analysis of DRH-3 will clarify the potentially novel mechanism by which it protects against X-ray-induced damage in C. elegans.