Immunohistochemical Analysis of REG Iα Expression in Ulcerative Colitis-Associated Neoplastic Lesions
Immunohistochemical Analysis of REG Iα Expression in Ulcerative Colitis-Associated Neoplastic Lesions
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DOI：
10.1159/000321808
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发表时间：
2011-01-01
期刊：
影响因子：
3.2
通讯作者：
Fujimori, Takahiro
中科院分区：
文献类型：
--
作者：
Tanaka, Hiroyuki;Fukui, Hirokazu;Fujimori, Takahiro
Background and Aims: The regenerating gene (REG) I alpha has been identified by microarray analysis as a gene that is distinctly overexpressed in ulcerative colitis (UC), and its protein product is suggested to play a pivotal role in the development of UC-associated carcinoma. In the present study, we investigated the significance of REG I alpha expression as a diagnostic marker of UC-associated neoplasia. Methods: Tissue samples were obtained from colectomy specimens from 31 patients with long-standing UC (mean disease duration 17.2 years, range 5-29). The lesions were evaluated according to the International Classification for Dysplasia in Inflammatory Bowel Diseases, and the sections were examined using immunohistochemistry for REG I alpha and p53. Results: In the 'regenerating atypia' group, REG I alpha immunoreactivity was restricted to the lower third of the UC mucosa (grade 1). Lesions classified as 'indefinite for dysplasia' also showed predominantly basal-type staining for REG I alpha. However, in 'low-grade dysplasia' and 'high-grade dysplasia' lesions, the localization of REG I alpha immunoreactivity expanded to the middle (grade 2) and upper (grade 3) third of the UC mucosa, respectively. The REG I alpha immunostaining pattern differed significantly (p < 0.0001) between non-neoplastic and neoplastic lesions, and was significantly (p < 0.0001) associated with p53 overexpression. Conclusions: Immunohistochemical analysis of REG I alpha expression is useful for differential diagnosis of nonneoplastic and neoplastic lesions in UC tissues. Copyright (C) 2011 S. Karger AG, Basel
