Identification and characterization of human TP53I5 and mouse Tp53i5 genes in silico.
Identification and characterization of human TP53I5 and mouse Tp53i5 genes in silico.
复制标题
人类 TP53I5 和小鼠 Tp53i5 基因的计算机鉴定和表征。
DOI：
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发表时间：
2004
影响因子：
5.2
通讯作者：
M. Katoh
中科院分区：
文献类型：
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作者：
Masuko Katoh;M. Katoh
TP 53基因编码肿瘤蛋白P53，在包括结肠直肠癌、肺癌和胃癌在内的多种人类癌症中经常失活。具有TP 53突变的癌细胞获得恶性潜能，例如选择性生长优势、基因组不稳定性、抗凋亡和促进血管生成。从HPV-18 E6/E7永生化角质形成细胞中分离的U33271部分cDNA来源于P53靶基因之一。本研究利用生物信息学方法鉴定了U33271部分cDNA对应的TP 53 I5基因。FLJ 23270（AK026923.1）是代表性的人TP 53 I5 cDNA。TP 53 I5基因定位于人类染色体11p15.5，由23个外显子组成。TP 53 I5 mRNA在人结直肠癌、肺癌和乳腺癌中表达。IMAGE 5352905（BC025477.1）和IMAGE 6508837（NM_178381.2）是由于内含子序列的保留而具有多个移码的异常小鼠Tp 5315 cDNA。通过组装BC025477.1的核苷酸位置1-1213、1293-1740、1745-2045和2071-3088来确定小鼠Tp 5315 cDNA的完整编码序列。TP 53 i5基因定位于小鼠7 F5染色体上。人TP 53 i5（782 aa）和小鼠TP 53 i5（747 aa）显示71.1%的总氨基酸同一性，是具有面向细胞质的N-和C-末端尾部的八个跨膜蛋白。TP 53 I5直向同源物与具有TM 16 H1、TM 16 H2和TM 16 H3结构域的TMEM 16 A（FLJ 10261或0 RA 0 V2）、TMEM 16 B、TMEM 16 C、TMEM 16 D、TMEM 16 E和TMEM 16 F同源。TP 53 I5是基于膜拓扑结构和TM 16 H1 -3结构域的TMEM 16家族的新成员。这是首次报道人和小鼠TP 53 i5基因。
TP53 gene, encoding tumor protein P53, is frequently inactivated in various types of human cancer, including colorectal, lung, and gastric cancer. Cancer cells with TP53 mutation acquire malignant potentials, such as selective growth advantage, genomic instability, resistance to apoptosis, and promotion of angiogenesis. U33271 partial cDNA isolated from HPV-18 E6/E7 immortalized keratinocytes is derived from one of P53-traget genes. Here, we identified and characterized the TP53I5 (tumor protein P53 inducible protein 5) gene corresponding to U33271 partial cDNA by using bioinformatics. FLJ23270 (AK026923.1) was the representative human TP53I5 cDNA. TP53I5 gene, consisting of 23 exons, was located at human chromosome 11p15.5. TP53I5 mRNA was expressed in human colorectal, lung, and breast cancer. IMAGE5352905 (BC025477.1) and IMAGE6508837 (NM_178381.2) were aberrant mouse Tp53i5 cDNAs with multiple frameshifts due to retention of intronic sequences. Complete coding sequence of mouse Tp53i5 cDNA was determined by assembling nucleotide positions 1-1213, 1293-1740, 1745-2045, and 2071-3088 of BC025477.1. Tp53i5 gene was located at mouse chromosome 7F5. Human TP53I5 (782 aa) and mouse Tp53i5 (747 aa), showing 71.1% total-amino-acid identity, were eight-transmembrane proteins with N- and C-terminal tails facing the cytoplasm. TP53I5 orthologs were homologous to TMEM16A (FLJ10261 or ORAOV2), TMEM16B, TMEM16C, TMEM16D, TMEM16E and TMEM16F with the TM16H1, TM16H2, and TM16H3 domains. TP53I5 was identified as a novel member of the TMEM16 family based on membrane topology and TM16H1-3 domains. This is the first report on the human TP53I5 and mouse Tp53i5 genes.