Complete genome sequence analysis of Paris alphapartitivirus 1: a novel member of the genus Alphapartitivirus infecting Paris polyphylla var. yunnanensis
Complete genome sequence analysis of Paris alphapartitivirus 1: a novel member of the genus Alphapartitivirus infecting Paris polyphylla var. yunnanensis
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DOI：
10.1007/s00705-022-05531-6
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发表时间：
2022-07
影响因子：
2.7
通讯作者：
Zhe Wang;R. F. Anane;Zeli Chen;Like Gao;Shangyun Li;Bifan Chu;Guosong Wen;Mingfu Zhao
中科院分区：
文献类型：
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作者：
Zhe Wang;R. F. Anane;Zeli Chen;Like Gao;Shangyun Li;Bifan Chu;Guosong Wen;Mingfu Zhao
从云南产的巴黎（Paris polyphyllavar. yunnanensis）叶片中检测到一种新的双链RNA病毒，暂命名为“巴黎花叶病毒1号”（ParAPV 1，OL 960006-OL 960007）。使用Illumina和桑格测序确定其完整的基因组序列。ParAPV 1具有由编码病毒RNA依赖性RNA聚合酶（RdRp）的dsRNA 1（1，917 bp）和编码推定的外壳蛋白（CP）的dsRNA 2（1，818 bp）组成的二分基因组。序列比较表明，ParAPV 1的RdRp和CP与梨花叶病毒（PpPV 2）的RdRp和CP的氨基酸序列同源性最高，分别为69.97%和54.21%。对ParAPV 1和其他partitivirus的RdRp氨基酸序列的系统发育分析表明，ParAPV 1与包含Parapartivirus的病毒在一个进化枝中聚类，并且其最近的已知亲缘关系是PpPV 2（BBA 66577）和玫瑰partitivirus（RoPV，ANQ 45203 S）。这些结果表明ParAPV 1应该被认为是Partitiviridae中Alphapartitivirus属的一个新成员。这是首次报道一种侵染云南多叶藓的分离病毒。
A novel double-stranded RNA (dsRNA) virus, tentatively named “Paris alphapartitivirus 1” (ParAPV1, OL960006-OL960007), was detected inParis polyphyllavar.yunnanensisplants exhibiting leaf chlorosis and shrinkage symptoms in Yunnan. Its complete genome sequence was determined using Illumina and Sanger sequencing. ParAPV1 has a bipartite genome that consists of dsRNA1 (1,917 bp) encoding the viral RNA-dependent RNA polymerase (RdRp), and dsRNA2 (1,818 bp) encoding the putative coat protein (CP). Sequence comparisons showed that the RdRp and CP of ParAPV1 are most similar to those of pear alphapartitivirus (PpPV2), with 69.97% and 54.21% amino acid sequence identities respectively. Phylogenetic analysis of the RdRp amino acid sequences of ParAPV1 and other partitiviruses showed that ParAPV1 cluster with viruses in a clade containing alphapartitiviruses, and that its closest known relatives are PpPV2 (BBA66577) and rose partitivirus (RoPV, ANQ45203S). Taken together, these results suggest that ParAPV1 should be regarded as a new member of genusAlphapartitivirusin the familyPartitiviridae. This is the first report of a partitivirus infectingP.polyphyllavar.yunnanensis.