Proteomic surveillance of autoantigens in patients with Behcet's disease by a proteomic approach
Proteomic surveillance of autoantigens in patients with Behcet's disease by a proteomic approach
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DOI：
10.1111/j.1348-0421.2010.00215.x
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发表时间：
2010-06-01
影响因子：
2.6
通讯作者：
Kato, Tomohiro
中科院分区：
文献类型：
--
作者：
Ooka, Seido;Nakano, Hiromasa;Kato, Tomohiro
为了促进对 BD 自身免疫的了解，我们调查了 BD 患者的自身抗原，并研究了已识别的自身抗体的患病率和临床意义。具体来说，使用 BD 患者的 5 个血清样本，对从外周血单核细胞中提取并通过 2DE 分离的蛋白质进行 WB。通过质谱法鉴定检测到的候选自身抗原。结果，2DE-WB检测到17个自身抗原点，其中鉴定出8个点。它们是 enolase-1、cofilin-1、波形蛋白、Rho-GDI β 蛋白、微管蛋白样蛋白和肌动蛋白样蛋白。 WB 使用重组蛋白对 30 名 BD 患者、35 名 RA 患者、32 名 SLE 患者和 16 名 PM/DM 患者的自身抗体进行了研究。通过WB在30名BD患者中的4名（13.3％）、35名RA患者中的5名（14.3％）、32名SLE患者中的2名（6.3％）以及33名PM/DM患者中的8名（24.2％）中检测到cofilin-1的自身抗体。我们的数据表明，cofilin-1 自身抗体的产生可能反映了 BD、RA 和 PM/DM 中常见的免疫紊乱。我们的数据将有助于理解 BD 的免疫病理学。此外，蛋白质组学方法将是研究自身抗原的有用方法。
To promote an understanding of autoimmunity in BD, we surveyed autoAgs in patients with BD and investigated the prevalence and clinical significance of the identified autoAbs. Specifically, proteins, extracted from peripheral blood mononuclear cells and separated by 2DE, were subjected to WB, using five serum samples from patients with BD. The detected candidate autoAgs were identified by mass spectrometry. As a result, 17 autoantigenic spots were detected by the 2DE-WB, out of which eight spots were identified. They are enolase-1, cofilin-1, vimentin, Rho-GDI beta protein, tubulin-like protein, and actin-like proteins. The autoAbs to one of the identified proteins, cofilin-1, were investigated by WB using a recombinant protein in 30 patients with BD, 35 patients with RA, 32 patients with SLE, and 16 patients with PM/DM. The autoAbs to cofilin-1 were detected by WB in four (13.3%) of the 30 patients with BD, five (14.3%) of the 35 patients with RA, two (6.3%) of the 32 patients with SLE, and eight (24.2%) of the 33 patients with PM/DM. Our data indicate that the generation of autoAbs to cofilin-1 may reflect common immunological disorders in BD, RA, and PM/DM. Our data would help understanding of the immunopathology of BD. In addition, the proteomic approach would be a useful way to investigate autoAgs.