A MEMBRANE FORM OF GUANYLATE-CYCLASE IS AN ATRIAL NATRIURETIC PEPTIDE RECEPTOR
A MEMBRANE FORM OF GUANYLATE-CYCLASE IS AN ATRIAL NATRIURETIC PEPTIDE RECEPTOR
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DOI：
10.1038/338078a0
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发表时间：
1989-03-02
期刊：
影响因子：
64.8
通讯作者：
SCHULZ, S
中科院分区：
文献类型：
--
作者：
CHINKERS, M;GARBERS, DL;SCHULZ, S
心房利钠肽（ANP）是一种多肽激素，其作用包括诱导利尿、利钠和血管舒张1。ANP与靶细胞上的受体结合后的最早事件之一是环GMP浓度的增加，这表明该核苷酸可能充当β 2，3的生理效应的介体。鸟苷酸环化酶至少以两种不同的分子形式存在：一种可溶性含血红素酶，由两个亚基组成4，5和一种不含血红素的跨膜蛋白，具有单个亚基6。它是膜形式的鸟苷酸环化酶，在ANP与靶细胞结合后被激活3，7，8。我们在这里报告的分离，测序和表达的互补DNA克隆编码膜形式的鸟苷酸环化酶从大鼠脑。将该cDNA转染到培养的哺乳动物细胞中，导致鸟苷酸环化酶活性和ANP结合活性的表达。ANP受体/鸟苷酸环化酶代表一类新的哺乳动物细胞表面受体，其含有胞外配体结合结构域和胞内鸟苷酸环化酶催化结构域。
ATRIAL natriuretic peptide (ANP) is a polypeptide hormone whose effects include the induction of diuresis, natriuresis and vasorelaxation1. One of the earliest events following binding of ANP to receptors on target cells is an increase in cyclic GMP concentration, indicating that this nucleotide might act as a mediator of the physiological effects of the hormone2,3. Guanylate cyclase exists in at least two different molecular forms: a soluble haem-containing enzyme consisting of two summits4,5and a non-haem-containing transmembrane protein having a single subunit6. It is the membrane form of guanylate cyclase that is activated following binding of ANP to target cells3,7,8. We report here the isolation, sequence and expression of a complementary DNA clone encoding the membrane form of guanylate cyclase from rat brain. Transfec-tion of this cDNA into cultured mammalian cells results in expression of guanylate cyclase activity and ANP-binding activity. The ANP receptor/guanylate cyclase represents a new class of mammalian cell-surface receptors which contain an extracellular ligand-binding domain and an intracellular guanylate cyclase catalytic domain.