Electrochemical Signal Enhancer Fabricated Using Lysine-Rich Peptide for Ultrasensitive Electrochemical DNA Biosensor Analysis
Electrochemical Signal Enhancer Fabricated Using Lysine-Rich Peptide for Ultrasensitive Electrochemical DNA Biosensor Analysis
复制标题
使用富含赖氨酸的肽制造电化学信号增强器，用于超灵敏电化学 DNA 生物传感器分析
DOI：
10.1002/elan.201800697
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发表时间：
2019
期刊：
影响因子：
3
通讯作者：
Yifu Guan
中科院分区：
文献类型：
--
作者：
Ying Yuan;Zhe Xun;Bin Zhang;Yifu Guan
Here we present a novel design of electrochemical signal enhancer to increase the detection sensitivity of electrochemical DNA biosensors. The key element of this enhancer is a lysine‐rich peptide (LRP). Its C‐terminal is conjugated with a planer molecule, being able to intercalate into the base pairs of probe‐target duplexes. The lysine residues of LRP are covalently linked with electrochemical signal indicators, acting as an assembly of electrochemical signal indicators. Experimental results proved the feasibility of the novel design. We have examined the effects of the numbers of lysine residues and the hybridization conditions on the detection sensitivity. The optimization procedures have led to significant sensitivity enhancement, and the LOD (limit of detection) has been determined to be 1.4 amol. This enhancer demonstrates advantages of easy operation, simple instrumentation, and high exemption from environmental influence.
