Flexible Electro‐Optical Arrays for Simultaneous Multi‐Site Colocalized Spatiotemporal Cardiac Mapping and Modulation
Flexible Electro‐Optical Arrays for Simultaneous Multi‐Site Colocalized Spatiotemporal Cardiac Mapping and Modulation
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DOI：
10.1002/adom.202201331
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发表时间：
2022-09
影响因子：
9
通讯作者：
Sofian N. Obaid;Zhiyuan Chen;Micah Madrid;Zexu Lin;Jinbi Tian;Camille Humphreys;Jillian Adams;Nicolas Daza;Jade Balansag;Igor R. Efimov;Luyao Lu
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作者：
Sofian N. Obaid;Zhiyuan Chen;Micah Madrid;Zexu Lin;Jinbi Tian;Camille Humphreys;Jillian Adams;Nicolas Daza;Jade Balansag;Igor R. Efimov;Luyao Lu
生物电子设备，允许同时准确的监测和控制的时空模式的心脏活动提供了一种有效的手段，了解机制和优化治疗策略的心脏病。由于其细胞类型选择性和高时空分辨率等优点，光遗传学是心脏研究的一项有前途的技术，但其有效性受到调制通道数量不足以及目前可用于体内研究的植入式心脏光遗传学工具缺乏同步时空映射能力的限制。在这里，软植入式电光心脏设备集成了多层高度均匀的透明微电极阵列和薄的柔性平台中的发光二极管，设计用于机械兼容的高含量高精度电标测和单/多位点光遗传学和电刺激，而没有光诱导伪影。系统的台架表征以及对健康和患病小动物心脏和人类心脏切片的离体和体内评价证明了其在真实的时空标测和心律与功能控制中的功能，在基础和最终临床心脏病学中具有广泛的应用。
Bioelectronic devices that allow simultaneous accurate monitoring and control of the spatiotemporal patterns of cardiac activity provide an effective means to understand the mechanisms and optimize therapeutic strategies for heart disease. Optogenetics is a promising technology for cardiac research due to its advantages such as cell‐type selectivity and high space‐time resolution, but its efficacy is limited by the insufficient number of modulation channels and lack of simultaneous spatiotemporal mapping capabilities in current implantable cardiac optogenetics tools available for in vivo investigations. Here, soft implantable electro‐optical cardiac devices integrating multilayered highly uniform arrays of transparent microelectrodes and multicolor light‐emitting diodes in thin, flexible platforms are designed for mechanically compliant high‐content high‐precision electrical mapping and single‐/multi‐site optogenetics and electrical stimulation without light‐induced artifacts. Systematic benchtop characterizations, together with ex vivo and in vivo evaluations on healthy and diseased small animal hearts and human cardiac slices demonstrate their functionalities in real‐time spatiotemporal mapping and control of cardiac rhythm and function, with broad applications in basic and ultimately clinical cardiology.