A Microplate Technique Employing a Gel Chamber Compared with Other Micro- and Macroplate Techniques for Immune Diffusion
A Microplate Technique Employing a Gel Chamber Compared with Other Micro- and Macroplate Techniques for Immune Diffusion
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采用凝胶室的微孔板技术与其他免疫扩散微孔板和大孔板技术的比较
DOI：
10.1159/000229325
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发表时间：
1962
影响因子：
2.8
通讯作者：
C. Wadsworth
中科院分区：
文献类型：
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作者：
C. Wadsworth
方法采用凝胶室显微技术。通过在一块有机玻璃（35 x40 mm）上钻孔（直径为3 mm，但在下表面具有2 mm的孔径）来制备基质，从而形成合适的图案。厚度（2-4 mm）这将提供所需的盆体积。将宽度不超过4 mm的防水胶带条施加到基质的下表面（在两个相对侧上）以提供所需深度（0.4- 0.8mm）的腔室。(If适当小心地处理，这样的基质可以使用多次）。清洁载玻片（50 x50 x 1 mm）涂布约0.2 ml琼脂，0.3%琼脂，并使其干燥。凝胶扩散室（见图1）是通过将基质置于琼脂薄膜载玻片上并使足量的含1%琼脂的0.85%NaCl溶液在50-60 ℃下从其中一个开口侧流入室中而形成的。如果在倒平板的过程中，琼脂被迫进入盆中，则建议将其移除一些
Methods The gel chamber microtechnique developed by the author. The matrix is prepared by drilling holes of 3 mm. in diameter but with an aperture at the lower surface of 2 mm. making a suitable pattern in a piece of plexiglass (35x40 mm.) of a thickness (2-4 mm.) which will provide the desired basin volume. Strips of waterproof tape not more than 4 mm. wide are applied to the lower surface (on two opposite sides) of the matrix to provide a chamber of the desired depth (0.4-0.8 mm.).(If handled with moderate care such a matrix may be used many times.) Cleansed glass slides (50x50x 1 mm.) arc agar filmed by spreading about 0.2 ml. of 0.3% agar over each and permitting it to dry. The gel diffusion chamber (see fig. 1) is formed by placing a matrix on an agar filmed slide and allowing a sufficient quantity of 1% agar in 0.85% NaCl at 50-60 C to flow into the chamber from one of the open sides. If, in the process of pouring the plate, the agar is forced up into the basins it is advisable to remove some of it