Engineering of avermectin biosynthetic genes to improve production of ivermectin in Streptomyces avermitilis
Engineering of avermectin biosynthetic genes to improve production of ivermectin in Streptomyces avermitilis
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DOI：
10.1016/j.bmcl.2008.09.061
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发表时间：
2008-10-15
影响因子：
2.7
通讯作者：
Li, Jilun
中科院分区：
文献类型：
--
作者：
Li, Meng;Chen, Zhi;Li, Jilun
通过在阿维链霉菌 73-12 中进行结构域和模块交换，构建了两种新的阿维菌素聚酮合酶重组体。然而，只有通过结构域取代形成的菌株S. avermitilis OI-31才能产生伊维菌素。对伊维菌素合成基因簇的分析表明，aveC转录本数量减少是导致伊维菌素产量低的因素之一。 aveC 的过表达可以提高伊维菌素的产量。 (C) 2008 Elsevier Ltd. 保留所有权利。
Two new recombinants of avermectin polyketide synthases were constructed by domain and module swapping in Streptomyces avermitilis 73-12. However, only the strain, S. avermitilis OI-31, formed by domain substitution could produce ivermectin. Analysis of the ivermectin synthesized gene cluster showed that decreased amount of aveC transcripts was one of the factors causing low yield of ivermectin. Overexpression of aveC could improve ivermectin yield. (C) 2008 Elsevier Ltd. All rights reserved.