Retrieval and amplification of single-copy genomic DNA from a nanometer region of chromosomes: a new and potential application of atomic force microscopy in genomic research.
Retrieval and amplification of single-copy genomic DNA from a nanometer region of chromosomes: a new and potential application of atomic force microscopy in genomic research.
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从染色体纳米区域检索和扩增单拷贝基因组 DNA：原子力显微镜在基因组研究中的新的潜在应用。
DOI：
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发表时间：
1998
期刊：
影响因子：
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通讯作者：
Atsushi Ikai
中科院分区：
文献类型：
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作者：
Xue;Atsushi Ikai
使用原子力显微镜（AFM）的改良尖端，我们从小鼠染色体的纳米区域收获了几条基因组DNA链。我们还共同开发了一种随机PCR方法来扩增回收的基因组DNA，其中一个单一的DNA分子的几个内切酶对可以有效地扩增。随后的荧光原位杂交（FISH）表明，扩增的DNA最初来自小鼠染色体的尖端操作区域。将PCR产物亚克隆到pUC 18质粒中，Southern杂交鉴定出几个含有独特序列的片段。本研究结果显示了原子力显微镜在基因组分析中的潜在应用。
Using a modified tip of the atomic force microscope (AFM), we harvested several strands of genomic DNA from a nanometer region of mouse chromosomes. We have also co-developed a random PCR method to amplify the recovered genomic DNA, in which a single DNA molecule of several kilobasepairs could be amplified efficiently. A subsequent fluorescence in situ hybridization (FISH) indicated that the amplified DNA originally came from the tip-manipulated regions of mouse chromosomes. Several fragments containing unique sequences were identified using Southern hybridization after subcloning the PCR products into pUC18 plasmid. The present results showed a potential application of AFM to genomic analysis.
影响因子：
14.9
作者：
HENDERSON, E
通讯作者：
HENDERSON, E